caa a22 4500 465824099 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164521 doi (NATIONALLICENCE)springer-10.1007/BF00164521 Inactivation of lytic enzymes by heat treatment in ultrafiltration-coupled acetonobutylic fermentation [Elektronische Daten] [M. Minier, P. Soucaille, E. Blanc-Ferras, A. Izard, G. Goma] Summary: Clostridium acetobutylicum cultures were grown in batch mode, using mineral ultrafiltration membranes to confine the microbial cells in the fermentor while the permeate was subject to heat treatment (70° C for 20 min) before recycling. The extracellular lytic activity in the broth, measured by the initial rate of lysis of a buffered suspension of Clostridium cell walls, was strongly decreased by a factor of 6.7 at pH 5.5, and 3.9 at pH 4.8. This caused an enhancement of the growth rate during the course of fermentation and an increase in the maximal cell concentration, which was practically doubled in heat-treatment assays. The solvent production results obtained were less positive in terms of productivity and final butanol concentration, since there was no significant improvement, despite elimination of lytic enzyme inhibition. The heat-inactivation of lytic enzymes influenced the culture more than the suppression of product inhibition, particularly concerning the activity and orientation of this bacterial metabolism. Springer-Verlag, 1990 Minier M. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut Soucaille P. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut Blanc-Ferras E. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut Izard A. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut Goma G. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 274-279 0175-7598 33:3<274 1990 33 253 https://doi.org/10.1007/BF00164521 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164521 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Minier M. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut NATIONALLICENCE 700 1- Soucaille P. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut NATIONALLICENCE 700 1- Blanc-Ferras E. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut NATIONALLICENCE 700 1- Izard A. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut NATIONALLICENCE 700 1- Goma G. Départment de Génie Biochimique et Alimentaire, UA-CNRS-544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 274-279 0175-7598 33:3<274 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer