caa a22 4500 465824102 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164532 doi (NATIONALLICENCE)springer-10.1007/BF00164532 Manganese inhibition of 3-acetyldeoxynivalenol biosynthesis in Fusarium graminearum R 2118 [Elektronische Daten] [Amit Vasavada, Dennis Hsieh] Summary: Biosynthesis of 3-acetyldeoxynivalenol (3-ADN) by Fusarium graminearum R 2118 was inhibited in the presence of manganese (Mn+2) at 10−3 to 50 mM in production medium. In this concentration range, toxin production was reduced by as much as 92%, while mycelial dry weight increased by as much as 50%. A rapid decline in pH accompanied the increased mycelial growth and intracellular accumulation of a yellow pigment. This inhibitory effect of Mn+2 on 3-ADN production was reversed by the presence of sodium citrate, sodium pyruvate, sodium acetate, or the chelating agent ethylenediaminetetraacetate (EDTA) in the medium but not by the presence of other organic acids of the tricarboxylic acid cycle or the protein synthesis inhibitors, chloramphenicol and cycloheximide. Evidence indicates that citric acid was playing a crucial role in 3-ADN biosynthesis and that Mn+2 was acting on the isocitrate dehydrogenase and acetyl coenzyme-A carboxylase sites to inhibit the toxin biosynthesis. Springer-Verlag, 1990 Vasavada Amit Department of Environmental Toxicology, University of California, 95616, Davis, CA, USA aut Hsieh Dennis Department of Environmental Toxicology, University of California, 95616, Davis, CA, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 335-339 0175-7598 33:3<335 1990 33 253 https://doi.org/10.1007/BF00164532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vasavada Amit Department of Environmental Toxicology, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Hsieh Dennis Department of Environmental Toxicology, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 335-339 0175-7598 33:3<335 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer