caa a22 4500 465824110 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164528 doi (NATIONALLICENCE)springer-10.1007/BF00164528 Genetic transformation of a mycorrhizal fungus [Elektronische Daten] [Victoria Barrett, Robert Dixon, Paul Lemke] Summary: Protoplasts of the ectomycorrhizal fungus, Laccaria laccata, were transformed at frequencies of 5-50 transformants/μg DNA. Transformation was based on positive selection for resistance to hygromycin B (HmB) using the Escherichia coli aminocyclitol phosphotransferase (aph) gene bracketed by an Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter and the transcription terminator region of the A. nidulans tryptophan synthetase (trpC) gene. Southern blot hybridization revealed that HmB-resistant transformants of L. laccata have integrated vector sequences involving one or more recombination events. These results provide the first evidence for genetic transformation of a mycorrhizal fungus and further indicate the ability of promoter and termination signals of ascomycetous origin to function in a taxonomically unrelated basidiomycete. The potential to improve ectomycorrhizal symbiosis through transgenic manipulation of the fungal component can now be realized, provided specific genes beneficial to this symbiosis can be identified. Springer-Verlag, 1990 Barrett Victoria Department of Botany and Microbiology, Auburn University, 36849, Auburn, AL, USA aut Dixon Robert School of Forestry and Alabama Agricultural Experiment Station, Auburn University, 36849, Auburn, AL, USA aut Lemke Paul Department of Botany and Microbiology, Auburn University, 36849, Auburn, AL, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 313-316 0175-7598 33:3<313 1990 33 253 https://doi.org/10.1007/BF00164528 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164528 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barrett Victoria Department of Botany and Microbiology, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Dixon Robert School of Forestry and Alabama Agricultural Experiment Station, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Lemke Paul Department of Botany and Microbiology, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 313-316 0175-7598 33:3<313 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer