caa a22 4500 465824145 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164534 doi (NATIONALLICENCE)springer-10.1007/BF00164534 Feruloyl and p -coumaroyl esterase from anaerobic fungi in relation to plant cell wall degradation [Elektronische Daten] [William Borneman, Roy Hartley, W. Morrison, Danny Akin, Lars Ljungdahl] Summary: Trans-feruloyl and trans-p-coumaroyl esterases were found in the culture filtrates of two monocentric (Piromyces MC-1, Neocallimastix MC-2) and three polycentric (Orpinomyces PC-2, Orpinomyces PC-3, and PC-1, an unnamed genus with uniflagellated zoospores) isolates of anaerobic rumen fungi. Treatment of cell walls of Coastal bermudagrass shoots with the filtrates released the trans isomers of ferulic and p-coumaric acids; results of microscopic observations indicated that fungal isolates degraded primarily unlignified cell walls in leaf blades and stems. A greater proportion of ferulic than p-coumaric acid was released by this treatment when compared with the amounts of the acids released by saponification of the walls with 1 M NaOH. The filtrates also showed esterase activities against the trans isomers of methyl ferulate and methyl p-coumarate, with ferulic acid being released at a faster rate than p-coumaric acid. Assays for other cell-wall-degrading enzymes (xylanase, β-xylosidase, α-l-arabinosidase, cellulase, β-glucosidase) indicated that only β-xylosidase correlated with ferulate and p-coumarate esterase activities. The monocentric isolate MC-2 had the highest esterase activity against both the plant cell wall and methyl ester substrates and the highest specific activities of acetyl esterase, β-xylosidase, α-l-arabinosidase, cellulase and β-glucosidase. Isolate MC-2 produced substantially greater amounts of feruloyl and p-coumaroyl esterase when the growth substrate contained higher levels of saponifiable ferulic and p-coumaric acids. Springer-Verlag, 1990 Borneman William Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut Hartley Roy Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut Morrison W. Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut Akin Danny Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut Ljungdahl Lars Center for Biological Resource Recovery and Department of Biochemistry, University of Georgia, 30602, Athens, Georgia, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 345-351 0175-7598 33:3<345 1990 33 253 https://doi.org/10.1007/BF00164534 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164534 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borneman William Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Hartley Roy Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Morrison W. Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Akin Danny Richard B. Russell Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, 30613, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Ljungdahl Lars Center for Biological Resource Recovery and Department of Biochemistry, University of Georgia, 30602, Athens, Georgia, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 345-351 0175-7598 33:3<345 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer