caa a22 4500 465824153 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164517 doi (NATIONALLICENCE)springer-10.1007/BF00164517 Interesterification of phosphatidylcholine with lipases in organic media [Elektronische Daten] [Ingemar Svensson, Patrick Adlercreutz, Bo Mattiasson] Summary: Lipases were investigated with respect to their ability to catalyse the incorporation of fatty acids into phosphatidylcholine (PC) by interesterification reactions. The enzymes were dried onto solid support materials and the conversions were carried out in water-saturated toluene. Three lipases (two fungal and one plant enzyme) had the desired activity; immobilized lipase from Mucor miehei (Lipozyme) was the most active enzyme. The Lipozyme-catalysed interesterification was selective for the sn-1 position of PC and during 48 h of reaction around 50% of the fatty acids in this position were replaced with heptadecanoic acid, a fatty acid which was practically absent in the original phospholipid. Due to adsorption on the support material and the competing hydrolysis reaction the total amount of PC in the reaction solution decreased to about 40% of the original amount. Higher interesterification rates were obtained with free fatty acids as acyl donors than with fatty acid esters. Springer-Verlag, 1990 Svensson Ingemar Department of Biotechnology, Chemical Center, University of Lund, P. O. Box 124, S-221 00, Lund, Sweden aut Adlercreutz Patrick Department of Biotechnology, Chemical Center, University of Lund, P. O. Box 124, S-221 00, Lund, Sweden aut Mattiasson Bo Department of Biotechnology, Chemical Center, University of Lund, P. O. Box 124, S-221 00, Lund, Sweden aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 255-258 0175-7598 33:3<255 1990 33 253 https://doi.org/10.1007/BF00164517 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164517 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Svensson Ingemar Department of Biotechnology, Chemical Center, University of Lund, P. O. Box 124, S-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Adlercreutz Patrick Department of Biotechnology, Chemical Center, University of Lund, P. O. Box 124, S-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Mattiasson Bo Department of Biotechnology, Chemical Center, University of Lund, P. O. Box 124, S-221 00, Lund, Sweden aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 255-258 0175-7598 33:3<255 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer