caa a22 4500 46582417X CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164523 doi (NATIONALLICENCE)springer-10.1007/BF00164523 Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment [Elektronische Daten] [Taku Matsushita, Masayoshi Ketayama, Ken-ichi Kamihata, Kazumori Funatsu] Summary: Anchorage-dependent mammalian cells were cultivated at high cell density in a novel culture system using polyurethane foam (PUF) as a substratum for cell attachment. PUF has a macroporous structure giving a high surface area to volume ratio. Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of PUF and grew to a high cell density (1.04 × 108 cells/ cm3 PUF and 3.5 × 107 cells/ cm3 PUF, respectively) in PUF stationary cultures. In addition, we have designed a PUF-particle packed-bed culture system for high density mass cell culture. A maximum cell density of 2.4 × 107 cells/cm3 culture vessel volume was obtained in a packed-bed culture of Vero cells. Springer-Verlag, 1990 Matsushita Taku Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut Ketayama Masayoshi Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut Kamihata Ken-ichi Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut Funatsu Kazumori Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 287-290 0175-7598 33:3<287 1990 33 253 https://doi.org/10.1007/BF00164523 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164523 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsushita Taku Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ketayama Masayoshi Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kamihata Ken-ichi Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Funatsu Kazumori Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 287-290 0175-7598 33:3<287 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer