caa a22 4500 465824188 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164525 doi (NATIONALLICENCE)springer-10.1007/BF00164525 Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans [Elektronische Daten] [Arnulf Timm, David Byrom, Alexander Steinbüchel] Summary: Recombinant strains of Pseudomonas oleovorans, which harbour the poly(3-hydroxybutyrate)-biosynthetic genes of Alcaligenes eutrophus, accumulated poly(hydroxyalkanoates), composed of 3-hydroxybutyrate(3HB), 3-hydroxyhexanoate (3HHx) and 3-hydroxyactanoate (3HO), up to 70% of the cell dry weight if the cells were cultivated with sodium octanoate as the carbon source. Physiological and chemical analysis revealed multiple evidence that this polymer is a blend of the homopolyester poly(3HB) and of the copolyester poly(3HHx-co-3HO) rather than a random or a block copolyester of 3HB, 3HHx and 3HO. The molar ratio between poly(3HHx-co-3HO) and poly(3HB) varied drastically during the process of fermentation. Whereas synthesis of poly(3HHx-co-3HO) started immediately after ammonium was exhausted in the medium, synthesis of poly(3HB) occurred only after a lag-phase. From freeze-dried cells poly(3HHx-co-3HO) was much more readily extracted with chloroform than was poly(3HB). The blend was fractionated into petrol-ether-insoluble poly(3HB) and petrol-ether-soluble poly(3HHx-co-3HO). The molecular weight values of these polyesters measured by gel permeation chromatography were 2.96 × 106 and 0.35 × 106 and were similar of those polymers accumulated by A. eutrophus or by wild-type P. oleovorans, respectively. Springer-Verlag, 1990 Timm Arnulf Institut für Mikrobiologie der Georg-August-Universität Göttinge3, Grisebachstrasse 8, D-Göttingen, Federal Republic of Germany aut Byrom David Imperial Chemical Industries, P. O. Box 1, TS23 1LB, Billingham, Cleveland, UK aut Steinbüchel Alexander Institut für Mikrobiologie der Georg-August-Universität Göttinge3, Grisebachstrasse 8, D-Göttingen, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 296-301 0175-7598 33:3<296 1990 33 253 https://doi.org/10.1007/BF00164525 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164525 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Timm Arnulf Institut für Mikrobiologie der Georg-August-Universität Göttinge3, Grisebachstrasse 8, D-Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Byrom David Imperial Chemical Industries, P. O. Box 1, TS23 1LB, Billingham, Cleveland, UK aut NATIONALLICENCE 700 1- Steinbüchel Alexander Institut für Mikrobiologie der Georg-August-Universität Göttinge3, Grisebachstrasse 8, D-Göttingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 296-301 0175-7598 33:3<296 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer