caa a22 4500 465824196 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164533 doi (NATIONALLICENCE)springer-10.1007/BF00164533 Physiological and enzymatic characterization of a novel pullulan-degrading thermophilic Bacillus strain 3183 [Elektronische Daten] [Gwo-Jenn Shen, Kailash Srivastava, Badal Saha, J. Zeikus] Summary: A new thermophilic Bacillus strain 3183 (ATCC 49341) was isolated from hot-spring sediments. The organism grew on pullulan as a carbon source and showed optimum pH and temperature at pH 5.5 and 62° C, respectively, for growth. The strain reduced nitrate to nitrite both aerobically and anaerobically. It produced extracellular thermostable pullulanase and saccharidase activities which degraded pullulan and starch into maltotriose, maltose, and glucose. Medium growth conditions for pullulanase production were optimized. The optimum pH and temperature for pullulanase activity were at pH 6.0 and 75° C, respectively. The enzyme was stable at pH 5.5-7.0 and temperature up to 70° C in the absence of substrate. The K m for pullulan at pH 6.0 and 75° C was 0.4 mg/ml. The pullulanase activity was stimulated and stabilized by Ca2+. It was inhibited by ethylenediaminetetraacetate (EDTA), beta and gamma-cyclodextrins but not by alpha-cyclodextrin and reagents that inhibit essential enzyme SH-groups. Springer-Verlag, 1990 Shen Gwo-Jenn Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut Srivastava Kailash Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut Saha Badal Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut Zeikus J. Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 340-344 0175-7598 33:3<340 1990 33 253 https://doi.org/10.1007/BF00164533 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164533 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Gwo-Jenn Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut NATIONALLICENCE 700 1- Srivastava Kailash Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut NATIONALLICENCE 700 1- Saha Badal Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut NATIONALLICENCE 700 1- Zeikus J. Michigan Biotechnology Institute, 3900 Collins Road, 48910, Lansing, MI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 340-344 0175-7598 33:3<340 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer