caa a22 4500 46582420X CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164531 doi (NATIONALLICENCE)springer-10.1007/BF00164531 The inhibition of clavine biosynthesis by 5-fluorotryptophan; a useful tool for the study of regulatory and biosynthetic relationships in Claviceps [Elektronische Daten] [Sylvie Pa<zoutová, Vladimir K<ren, P<remysl Sajdl] Summary: Clavine alkaloid production in submerged cultures of various Claviceps strains was inhibited by 0.25 mM 5-fluorotryptophan (5-Ftrp). The inhibition was removed by washing the cultures, or by addition of 50 mM tryptophan. Lower doses of tryptophan (0.25-25.0 mM) were ineffective. Addition of cycloheximide and/or 8-hydroxyquinoline (8-HOQ) to be inhibited cultures 24 and 48 h prior washing increased the rate of alkaloid synthesis in washed cultures (superinduction) and the rate of clavine synthesis was proportional to the length of 8-HOQ pretreatment. The addition of 0.25 mM tryptophan in washed cultures also stimulated alkaloid synthesis, but only for 2-3 days, then production in the cultures without tryptophan was higher. Cultures of Claviceps strains 59 and EK-10 inhibited with 5-Ftrp were capable of converting chanoclavine into further clavine pathway products. 5-Fluorotryptophan probably acts as an inhibitor of the first enzyme of the clavine pathway. However, the superinduction suggests the presence of a labile regulatory protein controlling clavine biosynthesis. Springer-Verlag, 1990 Pa<zoutová Sylvie Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, 1083, Víde<nská, Czechoslovakia aut K<ren Vladimir Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, 1083, Víde<nská, Czechoslovakia aut Sajdl P<remysl Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, 1083, Víde<nská, Czechoslovakia aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 330-334 0175-7598 33:3<330 1990 33 253 https://doi.org/10.1007/BF00164531 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164531 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pa<zoutová Sylvie Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, 1083, Víde<nská, Czechoslovakia aut NATIONALLICENCE 700 1- K<ren Vladimir Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, 1083, Víde<nská, Czechoslovakia aut NATIONALLICENCE 700 1- Sajdl P<remysl Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, 1083, Víde<nská, Czechoslovakia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 330-334 0175-7598 33:3<330 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer