caa a22 4500 465824218 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164522 doi (NATIONALLICENCE)springer-10.1007/BF00164522 Effect of bioreactor configuration on substrate uptake by cell suspension cultures of the plant Eschscholtzia californica [Elektronische Daten] [Ronald Taticek, Murray Moo-Young, Raymond Legge] Summary: The uptake of carbohydrates and oxygen by cell suspension cultures of the plant Eschscholtzia californica (California poppy) was studied in relation to biomass production in shake flasks, a 1-1 stirred-tank bioreactor and a 1-1 pneumatically agitated bioreactor. The sequence of carbohydrate uptake was similar in all cases, with sucrose hydrolysis occurring followed by the preferential uptake of glucose. The uptake of fructose was found to be affected by the oxygen supply rate. Carbohydrate utilization occurred at a slower rate in the bioreactors. Apparent biomass yields, Y X/S, ranged from 0.42 to 0.50 g biomass/g carbohydrate, while true biomass yields, Y′ X/S, were about 0.69 g/g. The maintenance coefficient for carbohydrate, m S, ranged between 0.002 and 0.008 g/dry weight (DW) per hour. The maximum measured specific oxygen uptake rate was 0.56 mmol O2/g DW per hour and occurred early in the growth stage. The decline in specific uptake rate coincided with a decline in cell viability. The oxygen uptake rate was faster in shake flasks, corresponding to the higher growth rate obtained. The true growth yield on oxygen, Y′X/O2, was calculated to range from 0.83 to 1.23 g biomass/g O2, while the maintenance coefficient, mO2, ranged from 0.15 to 0.25 mmol O2/g DW per hour. The growth yields for oxygen determined from the stoichiometry of an elemental balance were within 10% of those calculated from experimental data. Springer-Verlag, 1990 Taticek Ronald Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Moo-Young Murray Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Legge Raymond Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 280-286 0175-7598 33:3<280 1990 33 253 https://doi.org/10.1007/BF00164522 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164522 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taticek Ronald Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 700 1- Moo-Young Murray Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 700 1- Legge Raymond Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 280-286 0175-7598 33:3<280 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465824218 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer