caa a22 4500 465824226 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164515 doi (NATIONALLICENCE)springer-10.1007/BF00164515 Sequential two-step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone by thermophilic bacteria [Elektronische Daten] [Kunio Nishii, Koji Sode, Isao Karube] Summary: The sequential two-step conversion of 4-oxoisophorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) via diyhdrooxoisophorone (DOIP) was achieved using two kinds of thermophiles, Thermomonospora curvata and Bacillus stearothermophilus. In the first step, 83% OIP was converted to DOIP by T. curvata during 12 h incubation at 50° C. The resulting reaction mixture containing thermophile cells, DOIP (2.5 mg/ml), and OIP (0.5 mg/ml) was used directly in the second step after adjusting to pH 7 and adding glycerol. In the second step, DOIP in the reaction mixture was converted to 4-HTMCH by B. stearothermophilus. The final concentration of 4-HTMCH and DOIP after 24 h of incubation was 2.5 mg/ml and 0.5 mg/ml respectively; OIP was not detected. The total conversion yield of 4-HTMCH from OIP was 83% through the two-step conversion. The two-step conversion by a sequential culture system using T. curvata and B. stearothermophilus was found to be suitable for 4-HTMCH production. Springer-Verlag, 1990 Nishii Kunio Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., 31-12 Shimo 3-Chome, 115, Kitaku Toyko, Japan aut Sode Koji Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, 153, Meguro-ku Tokyo, Japan aut Karube Isao Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, 153, Meguro-ku Tokyo, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 245-250 0175-7598 33:3<245 1990 33 253 https://doi.org/10.1007/BF00164515 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164515 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishii Kunio Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., 31-12 Shimo 3-Chome, 115, Kitaku Toyko, Japan aut NATIONALLICENCE 700 1- Sode Koji Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, 153, Meguro-ku Tokyo, Japan aut NATIONALLICENCE 700 1- Karube Isao Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, 153, Meguro-ku Tokyo, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 245-250 0175-7598 33:3<245 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer