caa a22 4500 465824250 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176648 doi (NATIONALLICENCE)springer-10.1007/BF00176648 Lipase production by free and immobilized protoplasts of Sporotrichum (Chrysosporium) thermophile Apinis [Elektronische Daten] [B. Johri, J. Alurralde, J. Klein] Summary: Production of lipase by free and alginate-entrapped protoplasts was studied in batch culture. Cell-wall-degrading enzymes Novozym 234 and cellulase CP improved lipase secretion of normal mycelium by 25%-100%. The protoplast-regenerated mycelium exhibited several-fold higher lipase activity in batch replacements in TRIS buffer over normal spore-derived mycelium. The specific lipase activity of immobilized protoplasts was about four times higher than normal mycelial beads. Protoplasts beads were stable and retained high enzyme activity even after three buffer replacements lasting 120 h; TRIS buffer was better than acetate or normal glucose medium. A minimum of 8 h regeneration period was necessary for lipase synthesis. Triolein, olive oil, tributyrin and oleic acid butylester were able to induce lipase in immobilized protoplasts. Tween 80 enhanced lipase activity of the immobilized protoplasts. Partially degraded immobilized mycelium was nearly as effective as normal immobilized protoplasts for lipase secretion. Both free and immobilized protoplasts could be reused for up to 200 h with some loss in enzyme activity. Springer-Verlag, 1990 Johri B. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Alurralde J. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Klein J. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 367-371 0175-7598 33:4<367 1990 33 253 https://doi.org/10.1007/BF00176648 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176648 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johri B. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Alurralde J. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Klein J. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 367-371 0175-7598 33:4<367 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer