caa a22 4500 465824269 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176659 doi (NATIONALLICENCE)springer-10.1007/BF00176659 Production and characterization of human gamma interferon from Escherichia coli [Elektronische Daten] [L. Perez, J. Vega, C. Chuay, A. Menendez, R. Ubieta, M. Montero, G. Padron, A. Silva, C. Santizo, V. Besada, L. Herrera] Summary: The production of human gamma interferon as intracellular inclusion bodies in Escherichia coli, which simplified the purification process, is described. An expression plasmid carrying lipoprotein and the tryptophane promoters in tandem was used. Preparation of highly pure interferon was achieved using high resolution chromatography after denaturation and renaturation steps. Structural characteristics of this protein were verified by mass spectrometric analysis. Additional control tests have shown the suitability of the final product for clinical purposes. Springer-Verlag, 1990 Perez L. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Vega J. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Chuay C. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Menendez A. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Ubieta R. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Montero M. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Padron G. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Silva A. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Santizo C. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Besada V. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Herrera L. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 429-434 0175-7598 33:4<429 1990 33 253 https://doi.org/10.1007/BF00176659 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176659 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Perez L. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Vega J. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Chuay C. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Menendez A. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Ubieta R. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Montero M. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Padron G. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Silva A. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Santizo C. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Besada V. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 700 1- Herrera L. Center for Genetic Engineering and Biotechnology (CIGB), P. O. Box 6162, Havana, Cuba aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 429-434 0175-7598 33:4<429 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer