caa a22 4500 465824277 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176658 doi (NATIONALLICENCE)springer-10.1007/BF00176658 A Constitutive expression vector system driven by the deo P1P2 promoters of Escherichia coli [Elektronische Daten] [M. Fischer, S. Fytlovitch, B. Amit, A. Wortzel, Y. Beck] Summary: The P1P2 promoters of Escherichia coli K12 deo operon, residing on an AvaII restriction fragment, were used to construct a new expression vector. To evaluate the potential of the P1P2-driven expression system we have inserted the sequence of human superoxide dismutase (hSOD) downstream of the deo ribosome binding site. Expression of hSOD was evaluated by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis and enzyme activity. In crude cell extracts hSOD expression levels were found to be high in hosts possessing no deoR or cytR repressors. Highest levels of hSOD expression were obtained with a high-copy-number plasmid regardless of the host used. Expressed hSOD can account for 35%-40% of total protein in E. coli. Springer-Verlag, 1990 Fischer M. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut Fytlovitch S. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut Amit B. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut Wortzel A. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut Beck Y. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 424-428 0175-7598 33:4<424 1990 33 253 https://doi.org/10.1007/BF00176658 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176658 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fischer M. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut NATIONALLICENCE 700 1- Fytlovitch S. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut NATIONALLICENCE 700 1- Amit B. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut NATIONALLICENCE 700 1- Wortzel A. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut NATIONALLICENCE 700 1- Beck Y. Department of Molecular Biology, Biotechnology General Inc., Kiryat Weizmann, 76326, Rehovot, Israel aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 424-428 0175-7598 33:4<424 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer