caa a22 4500 465824285 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176664 doi (NATIONALLICENCE)springer-10.1007/BF00176664 Streptomycetes excreting dd -carboxypeptidases [Elektronische Daten] [W. Kurzątkowski, J. Solecka, J. Filipek, J. Kurzątkowski, W. Kuryłowicz] Summary: Excretion of exocellular dd-carboxypeptidases was tested using 128 strains of streptomycetes. Exocellular enzyme activity was shown in 13% of the trains investigated. Streptomyces strains showed low activity of excretion of dd-carboxypeptidases: 2.7-4.8 μM of released C-terminal d-alanine (d-Ala) residue/1 culture supernatant per minute. Saccharopolyspora erythraea mutants produced considerably higher levels of exocellular enzymes, the dynamics of excretion depending upon the medium used. The highest activity of exocellular dd-carboxypeptidase production was 44 μM d-Ala/1 culture supernatant per minute. The affinity of exocellular dd-carboxypeptidase of S. erythraea 64-575 for β-lactam antibiotics was assessed by a statistical computer programme. The enzyme showed the lowest affinity for sodium cefotaxime, ID50(M) = 7.5 × 10−6, and the highest for potassium cephalosporin C, ID50(M) = 5.0 × 10−9, ID50(M) representing the molar concentration of β-lactan antibiotics which decreased by 50% the release of d-Ala. Springer-Verlag, 1990 Kurzątkowski W. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut Solecka J. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut Filipek J. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut Kurzątkowski J. Pharmaceutical Faculty, Medical Academy of Warsaw, Banacha 1, 02-097, Warsaw, Poland aut Kuryłowicz W. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 452-454 0175-7598 33:4<452 1990 33 253 https://doi.org/10.1007/BF00176664 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176664 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurzątkowski W. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut NATIONALLICENCE 700 1- Solecka J. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut NATIONALLICENCE 700 1- Filipek J. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut NATIONALLICENCE 700 1- Kurzątkowski J. Pharmaceutical Faculty, Medical Academy of Warsaw, Banacha 1, 02-097, Warsaw, Poland aut NATIONALLICENCE 700 1- Kuryłowicz W. State Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 452-454 0175-7598 33:4<452 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer