caa a22 4500 465824323 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176651 doi (NATIONALLICENCE)springer-10.1007/BF00176651 d - p -Hydroxyphenylglycine production from dl -5-p-hydroxyphenylhydantoin by Agrobacterium sp [Elektronische Daten] [Serge Runser, Nicolas Chinski, Eric Ohleyer] Summary: A bacterium that stereospecifically produces D-p-hydroxyphenylglycine (D-PHPG) from DL-5-p-hydroxyphenylhydantoin (DL-5-PHPH) was isolated from soil and identified as Agrobacterium sp. IP-I 671. The hydantoinase and the N-carbamyl-amino acid amido-hydrolase involved in this biotransformation process were both strictly D-stereospecific. Their biosynthesis was found to be inducible by addition of 2-thiouracil to the cultivation media, or to a lesser extent by uracil. The amidohydrolase activity of Agrobacterium sp. was strongly inhibited by ammonium ions co-produced with D-PHPG, whereas the hydantoinase activity under the same conditions was unaffected. Optimum temperature and pH were respectively 55° C and 10 for the partially purified hydantoinase, 45° and 6.75 when resting cells were used. Biotransformation under these slightly acidic conditions allowed to complete conversion of 30 g/1 DL-5-PHPH into 25 g/l of D-PHPG (molar yield 96%) and involved enzymatic racemization of DL-5-PHPH. Springer-Verlag, 1990 Runser Serge Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91 198, Gif-Sur-Yvette, France aut Chinski Nicolas Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91 198, Gif-Sur-Yvette, France aut Ohleyer Eric Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91 198, Gif-Sur-Yvette, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 382-388 0175-7598 33:4<382 1990 33 253 https://doi.org/10.1007/BF00176651 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176651 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Runser Serge Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91 198, Gif-Sur-Yvette, France aut NATIONALLICENCE 700 1- Chinski Nicolas Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91 198, Gif-Sur-Yvette, France aut NATIONALLICENCE 700 1- Ohleyer Eric Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91 198, Gif-Sur-Yvette, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 382-388 0175-7598 33:4<382 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer