caa a22 4500 46582434X CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176655 doi (NATIONALLICENCE)springer-10.1007/BF00176655 Enhancement and stabilization of the production of glucoamylase by immobilized cells of Aureobasidium pullulans in a fluidized-bed reactor [Elektronische Daten] [Federico Federici, Maurizio Petruccioli, Martin Miller] Summary: Glucoamylase production by Aureobasidium pollulans A-124 was compared in free-living cells, cells immobilized in calcium alginate gel beads aerated on a rotary shaker (agitation rate 150 rpm), and immobilized cells aerated in an air bubble column reactor. Fermentation conditions in the bioreactor were established for bead concentration, substrate (starch) concentration, calcium chloride addition to the fermentation medium, and rate of aeration. Production of glucoamylase was optimized at approximately 1.5 units of enzyme activity/ml medium in the bioreactor under the following conditions: aeration rate, 2.0 vol air per working volume of the bioreactor (280 ml) per minute; gel bead concentration, 30% of the working volume; substrate (starch) concentration, at 0.3% (w/v); addition of calcium chloride to the medium at a final concentration of 0.01 M. Productivity levels were stabilized through the equivalent of ten batches of medium with the original inoculum of immobilized beads. Springer-Verlag, 1990 Federici Federico Istituto di Microbiologia e Tecnologie Agrarie e Forestali, Università della Basilicata, Via N. Sauro 85, I-85 100, Potenza, Italy aut Petruccioli Maurizio Istituto di Microbiologia e Tecnologie Agrarie e Forestali, Università della Basilicata, Via N. Sauro 85, I-85 100, Potenza, Italy aut Miller Martin Department of Food Science and Technology, University of California, 95 616, Davis, California, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 407-409 0175-7598 33:4<407 1990 33 253 https://doi.org/10.1007/BF00176655 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176655 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Federici Federico Istituto di Microbiologia e Tecnologie Agrarie e Forestali, Università della Basilicata, Via N. Sauro 85, I-85 100, Potenza, Italy aut NATIONALLICENCE 700 1- Petruccioli Maurizio Istituto di Microbiologia e Tecnologie Agrarie e Forestali, Università della Basilicata, Via N. Sauro 85, I-85 100, Potenza, Italy aut NATIONALLICENCE 700 1- Miller Martin Department of Food Science and Technology, University of California, 95 616, Davis, California, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 407-409 0175-7598 33:4<407 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer