caa a22 4500 465824358 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176663 doi (NATIONALLICENCE)springer-10.1007/BF00176663 Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound [Elektronische Daten] [Nigel Kilby, Christopher Hunter] Summary: Beta vulgaris L. (red beetroot) cells from a suspension culture were sonicated with continuous wave ultrasound (1.02 MHz) for periods ranging from 10 s to 60 s. In general, cells sonicated for ≥ 30 s released vacuole-located pigment into the surrounding medium, whereas cells sonicated for < 30 s and control (unsonicated) cells released no pigment. Sonicated cells were viable as determined using fluorescein diacetate: microscopic examination of a selected population of sonicated cells did not show any signs of obvious structural damage. Sonicated cell samples were subcultured to re-establish actively growing, pigment-competent cell cultures. Cells from such cultures were available for further harvests of the pigment by sonication. For sonication periods ≥ 50 s, repeated cycles of sonication and incubation allowed pigments to be harvested from cells every 7 days during a 21-day culture period. Springer-Verlag, 1990 Kilby Nigel Department of Genetics, University of Cambridge, Tennis Court Road, CB2 3EH, Cambridge, UK aut Hunter Christopher Department of Science, Plant Science Group, Bristol Polytechnic, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 448-451 0175-7598 33:4<448 1990 33 253 https://doi.org/10.1007/BF00176663 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176663 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kilby Nigel Department of Genetics, University of Cambridge, Tennis Court Road, CB2 3EH, Cambridge, UK aut NATIONALLICENCE 700 1- Hunter Christopher Department of Science, Plant Science Group, Bristol Polytechnic, Coldharbour Lane, BS16 1QY, Frenchay, Bristol, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 448-451 0175-7598 33:4<448 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer