caa a22 4500 465824366 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176652 doi (NATIONALLICENCE)springer-10.1007/BF00176652 Production of cholera toxin subunit B by a mutant strain of Vibrio cholerae [Elektronische Daten] [Michele van de Walle, Raphael Fass, Joseph Shiloach] Summary: The B subunit (CTB) of cholera toxin (CT) can be used as a carrier protein for conjugate vaccines designed to elicit antipolysaccharide antibodies. A defined medium, AGM4, was designed to grow a high-producing mutant of Vibrio cholerae expressing only the B subunit of CT: V. cholerae 0395-NI. AGM4 contains four amino acids, asparagine, glutamic acid, arginine and serine, salts and a trace element solution. The carbon source is glucose. The fermentations performed in AGM4 indicated that CTB production paraleled the growth of the organism but that there was a maximal release of CTB during the stationary phase. There was a clear optimum of productivity at pH 8.0 and 30°C. The pH had an influence on CTB production and not only on its release. Analysis of the amino acids present in the medium showed a correlation between their consumption rates and CTB productivity. Springer-Verlag, 1990 van de Walle Michele Biotechnology Unit, LCDB, NIDDK, NIH, Building 6, Room B1-33, 20892, Bethesda, MD, USA aut Fass Raphael Biotechnology Unit, LCDB, NIDDK, NIH, Building 6, Room B1-33, 20892, Bethesda, MD, USA aut Shiloach Joseph Biotechnology Unit, LCDB, NIDDK, NIH, Building 6, Room B1-33, 20892, Bethesda, MD, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 389-394 0175-7598 33:4<389 1990 33 253 https://doi.org/10.1007/BF00176652 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176652 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van de Walle Michele Biotechnology Unit, LCDB, NIDDK, NIH, Building 6, Room B1-33, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Fass Raphael Biotechnology Unit, LCDB, NIDDK, NIH, Building 6, Room B1-33, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Shiloach Joseph Biotechnology Unit, LCDB, NIDDK, NIH, Building 6, Room B1-33, 20892, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 389-394 0175-7598 33:4<389 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer