caa a22 4500 465824374 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176649 doi (NATIONALLICENCE)springer-10.1007/BF00176649 Heterogeneous enzymatic esterification: analysis of the effect of water [Elektronische Daten] [J. Boyer, B. Gilot, R. Guiraud] Summary: An analysis of the effect of water during the enzymatic esterification reaction between a fatty acid and a fatty alcohol is presented in this article. Adding water at the beginning of the reaction enabled the initial rate to be greatly increased. The action of water was not instantaneous, even though enzyme hydration was rapid. The apparent order of the reaction was zero. If water generated during the reaction was not eliminated, there was a drastic reaction rate decrease, which we attribute to a phenomenon of phase separation. Depending on the experimental conditions, which result in the presence of greater or smaller quantities of water in the medium, a conversion of 0.3, 0.5 or 0.9 is reached within 1 h. Springer-Verlag, 1990 Boyer J. Département Génie Chimique Chemin de la loge, Institut Universitaire de Technologie A, F-31078, Toulouse Cedex, France aut Gilot B. Ecole Nationale Supérieure d'Ingénieurs de Génie Chimique, Chemin de la loge, F-31078, Toulouse Cedex, France aut Guiraud R. Ecole Nationale Supérieure d'Ingénieurs de Génie Chimique, Chemin de la loge, F-31078, Toulouse Cedex, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 372-376 0175-7598 33:4<372 1990 33 253 https://doi.org/10.1007/BF00176649 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176649 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boyer J. Département Génie Chimique Chemin de la loge, Institut Universitaire de Technologie A, F-31078, Toulouse Cedex, France aut NATIONALLICENCE 700 1- Gilot B. Ecole Nationale Supérieure d'Ingénieurs de Génie Chimique, Chemin de la loge, F-31078, Toulouse Cedex, France aut NATIONALLICENCE 700 1- Guiraud R. Ecole Nationale Supérieure d'Ingénieurs de Génie Chimique, Chemin de la loge, F-31078, Toulouse Cedex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 372-376 0175-7598 33:4<372 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer