caa a22 4500 465824412 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00176662 doi (NATIONALLICENCE)springer-10.1007/BF00176662 Storage and growth of neuroblastoma cells immobilized in calcium-alginate beads [Elektronische Daten] [Christian Tamponnet, Sylvie Boisseau, Pierre-Noël Lirsac, Jean-Noël Barbotin, Chantal Poujeol, Maurice Lievremont, Michel Simonneau] Summary: Mouse neuroblastoma cells (N18) were immobilized in calcium-alginate gel beads. Under standard culture conditions (37° C; 5% CO2), cell growth was observed inside the beads. The number of cells increased threefold during 7 days of culture with cell division and differentiation visualized by electron microscopy. Cell properties maintained after short-term storage (2-3 days at 4° C) included: (i) properties of voltage-dependent ionic channels tested by patch-clamp electrophysiological techniques; (ii) expression of cell-adhesion membrane proteins tested by immunohistochemistry (iii) morphological differentiation obtained by depletion of foetal calf serum in culture medium. The advantages of such an immobilization technique as applied to neurone cells are discussed. Springer-Verlag, 1990 Tamponnet Christian Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Ecole Normale Supérieure, Cachan, F-94235, Cachan Cedex, France aut Boisseau Sylvie Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, F-91198, Gif sur Yvette, France aut Lirsac Pierre-Noël Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Ecole Normale Supérieure, Cachan, F-94235, Cachan Cedex, France aut Barbotin Jean-Noël Laboratoire de Technologie Enzymatique, (CNRS), no. 523, BP 649, F-60206, Compiègne Cedex, France aut Poujeol Chantal Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, F-91198, Gif sur Yvette, France aut Lievremont Maurice Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Ecole Normale Supérieure, Cachan, F-94235, Cachan Cedex, France aut Simonneau Michel Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, F-91198, Gif sur Yvette, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 442-447 0175-7598 33:4<442 1990 33 253 https://doi.org/10.1007/BF00176662 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176662 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tamponnet Christian Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Ecole Normale Supérieure, Cachan, F-94235, Cachan Cedex, France aut NATIONALLICENCE 700 1- Boisseau Sylvie Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, F-91198, Gif sur Yvette, France aut NATIONALLICENCE 700 1- Lirsac Pierre-Noël Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Ecole Normale Supérieure, Cachan, F-94235, Cachan Cedex, France aut NATIONALLICENCE 700 1- Barbotin Jean-Noël Laboratoire de Technologie Enzymatique, (CNRS), no. 523, BP 649, F-60206, Compiègne Cedex, France aut NATIONALLICENCE 700 1- Poujeol Chantal Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, F-91198, Gif sur Yvette, France aut NATIONALLICENCE 700 1- Lievremont Maurice Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée, Ecole Normale Supérieure, Cachan, F-94235, Cachan Cedex, France aut NATIONALLICENCE 700 1- Simonneau Michel Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, F-91198, Gif sur Yvette, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/4(1990-07-01), 442-447 0175-7598 33:4<442 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer