caa a22 4500 46582448X CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172544 doi (NATIONALLICENCE)springer-10.1007/BF00172544 Characterization of an alkaline protease from Bacillus sp. no. AH-101 [Elektronische Daten] [Hideto Takami, Teruhiko Akiba, Koki Horikoshi] Summary: The Bacillus sp. no. AH-101 alkaline protease showed higher hydrolysing activity against insoluble fibrous natural proteins such as elastin and keratin in comparison with subtilisins and Proteinase K. The optimum pH of the enzyme toward elastin and keratin was pH 10.5 and pH 11.0-12.0 respectively. The specific activity toward elastin and keratin was 10 600 units/mg protein and 3970 units/mg protein, respectively. The enzymatic activity was not inhibited by p-chloromercuribenzoic acid and iodoacetic acid. Carbobenzoxy-glycyl-glycyl-L-phenylalanyl chloromethyl ketone completely inhibited the caseinolytic activity, but 36% elastolytic activity remained. No inhibitory effect on caseinolytic and elastolytic activity was shown by tosyl-L-phenylalanyl-chloromethyl ketone, tosyl-L-lysine chloromethyl ketone, carbobenzoxy-L-phenylalanyl chloromethyl ketone, and elastatinal. The amino acid composition and amino terminal sequence of the enzyme were determined. The no. AH-101 alkaline protease was compared with subtilisin BPN', subtilisin Carlsberg, no. 221, and Ya-B alkaline proteases. Extensive sequence homology existed among these enzymes. Springer-Verlag, 1990 Takami Hideto Department of Microbial Ecology, The Institute of Physical and Chemical Research, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut Akiba Teruhiko Department of Microbial Ecology, The Institute of Physical and Chemical Research, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut Horikoshi Koki Department of Microbial Ecology, The Institute of Physical and Chemical Research, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 519-523 0175-7598 33:5<519 1990 33 253 https://doi.org/10.1007/BF00172544 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172544 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takami Hideto Department of Microbial Ecology, The Institute of Physical and Chemical Research, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 700 1- Akiba Teruhiko Department of Microbial Ecology, The Institute of Physical and Chemical Research, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 700 1- Horikoshi Koki Department of Microbial Ecology, The Institute of Physical and Chemical Research, Hirosawa 2-1, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 519-523 0175-7598 33:5<519 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer