caa a22 4500 465824501 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172558 doi (NATIONALLICENCE)springer-10.1007/BF00172558 Cryptic growth within a binary microbial culture [Elektronische Daten] [M. Banks, James Bryers] Summary: The ability of viable cells of the species Pseudomonas putida and Hyphomicrobium sp. to metabolize the particulate and soluble cellular organic constituents of both species was studied in a series of batch experiments. Both P. putida and Hyphomicrobium sp. were grown in individual batch reactors on either the 14C-labelled soluble or the particulate debris of sonicated cells of each species derived from steady-state chemostat cultures. Cell generation times (t g)observed for P. putida cultivated on soluble organic material originating from either sonicated P. putida or Hyphomicrobium sp. cells, were t g= 2.0 h and t g= 6.3 h, respectively. Corresponding t gvalues of Hyphomicrobium sp. on soluble organic material originating from sonicated P. putida and Hyphomicrobium so. were, respectively, 11.6 h and 4.3 h. While particulate debris originating from either species was solubilized by both P. putida and Hyphomicrobium sp., no increases in cell numbers were observed for either species. The data indicate that bacteria are capable of scavenging soluble material released upon cell lysis at near maximal rates; solubilization of debris also occurred but at much slower overall rates with no observable cell replication. The results reaffirm that cryptic growth and turnover of cellular biomass can be significant under situations of low substrate flux or starvation conditions. Springer-Verlag, 1990 Banks M. Department of Civil and Environmental Engineering, Duke University, 27706, Durham, NC, USA aut Bryers James The Center for Biochemical Engineering, Duke University, 27706, Durham, NC, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 596-601 0175-7598 33:5<596 1990 33 253 https://doi.org/10.1007/BF00172558 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172558 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banks M. Department of Civil and Environmental Engineering, Duke University, 27706, Durham, NC, USA aut NATIONALLICENCE 700 1- Bryers James The Center for Biochemical Engineering, Duke University, 27706, Durham, NC, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 596-601 0175-7598 33:5<596 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer