caa a22 4500 465824528 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172551 doi (NATIONALLICENCE)springer-10.1007/BF00172551 Stable transformation of cultured tobacco cells by DNA-coated gold particles accelerated by gas-pressure-driven particle gun [Elektronische Daten] [Asako Iida, Hiromichi Morikawa, Yasuzuki Yamada] Summary: Stable transformation of suspension-cultured cells of tobacco with plasmid DNA (pCaMV-NEO) harbouring the neomycin phosphotransferase II (npt-II) gene was achieved using a previously described gas-pressure-driven particle acceleration device. The cells were bombarded with DNA-coated gold particles accelerated by the device, and callus containing the introduced gene was selected in the presence of geneticin disulphate. The geneticin-resistant callus exhibited npt-II enzyme activity, and Southern analysis confirmed the stable integration of the foreign gene into the tobacco genome. Transformants were obtained at a rate of more than 1.9 × 10−4. Springer-Verlag, 1990 Iida Asako Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan aut Morikawa Hiromichi Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan aut Yamada Yasuzuki Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 560-563 0175-7598 33:5<560 1990 33 253 https://doi.org/10.1007/BF00172551 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172551 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iida Asako Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Morikawa Hiromichi Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamada Yasuzuki Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 560-563 0175-7598 33:5<560 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer