caa a22 4500 465824560 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172556 doi (NATIONALLICENCE)springer-10.1007/BF00172556 Kern Hartmut Forschungszentrum (KFA) Jülich, Institut für Biotechnologie, Postfach 1913, D-5170, Jülich 1, Federal Republic of Germany aut Production and stability of lignin peroxidases of Phanerochaete chrysosporium cultivated on glycerol in the presence of solid manganese(IV)oxide [Elektronische Daten] [Hartmut Kern] Summary: Lignin peroxidase production by Phanerochaete chrysosporium, under shaking conditions in an N-limited glycerol medium supplied with solid manganese(IV)oxide, increased to a high level. It was shown that the high enzymatic level was due to a higher specific enzymatic activity compared to corresponding −MnO2 cultures when measurements were based upon the haem component (A 409). The superiority of cultivation in the presence of MnO2 was reflected by the longevity of the enzymes produced in the culture fluid. By tracing enzymatic activities (toward veratryl alcohol and phenol red) as a function of time of incubation, a higher specific activity of single peroxidases from +MnO2 cultures was determined compared to corresponding −MnO2 cultures. Different patterns of peroxidases were found in glucose and glycerol cultures and the problems of classifying peroxidases are discussed. The effect of veratryl alcohol on peroxidases was compared with that of MmO2. Even at higher levels of enzymatic activity an additional influence of MnO2 on the stabilization of the enzymes was observed. By applying homoveratryl amine instead of veratryl alcohol the activity of the peroxidases in agitated +MnO2 cultures exceeded 2000 units/l. Springer-Verlag, 1990 Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 582-588 0175-7598 33:5<582 1990 33 253 https://doi.org/10.1007/BF00172556 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172556 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kern Hartmut Forschungszentrum (KFA) Jülich, Institut für Biotechnologie, Postfach 1913, D-5170, Jülich 1, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 582-588 0175-7598 33:5<582 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer