caa a22 4500 465824579 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172538 doi (NATIONALLICENCE)springer-10.1007/BF00172538 Effect of pH and acetic acid on growth and 2,3-butanediol production of Enterobacter aerogenes in continuous culture [Elektronische Daten] [An-Ping Zeng, Hanno Biebl, Wolf-Dieter Deckwer] Summary: The effect of pH and acetic acid on growth and 2,3-butanediol production of Enterobacter aerogenes from glucose was investigated in a microaerobic continuous culture. At a dilution rate of 0.20 h−1 and a fixed oxygen uptake rate (OUR) of 31.5 mmol l−1 h−1 the biomass concentration increased with pH ranging from 5.0 to 7.0, while the specific ATP requirement of the cells decreased. In the pH range 5.5-6.5 the product concentration (butanediol + acetoin) was maximal and nearly constant. However, the specific production continuously declined with increasing pH. Experiments with addition of acetic acid showed that the various effects of pH are due to inhibition of the by-product acetic acid on cell growth. The strength of the acetic and inhibition depended only on the concentration of its undissociated form [HAc]. The biomass concentration and the specific OUR were also only functions of [HAc], irrespective of the pH. Although the specific ATP requirement (q ATP) strongly depended on the pH, [HAc] at constant pH. Springer-Verlag, 1990 Zeng An-Ping Biochemical Engineering Division, Gesellschaft für Biotechnologische Forschung-National Research Institute for Biotechnology, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Biebl Hanno Biochemical Engineering Division, Gesellschaft für Biotechnologische Forschung-National Research Institute for Biotechnology, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Deckwer Wolf-Dieter Biochemical Engineering Division, Gesellschaft für Biotechnologische Forschung-National Research Institute for Biotechnology, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 485-489 0175-7598 33:5<485 1990 33 253 https://doi.org/10.1007/BF00172538 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172538 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng An-Ping Biochemical Engineering Division, Gesellschaft für Biotechnologische Forschung-National Research Institute for Biotechnology, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Biebl Hanno Biochemical Engineering Division, Gesellschaft für Biotechnologische Forschung-National Research Institute for Biotechnology, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Deckwer Wolf-Dieter Biochemical Engineering Division, Gesellschaft für Biotechnologische Forschung-National Research Institute for Biotechnology, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 485-489 0175-7598 33:5<485 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer