caa a22 4500 465824587 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172557 doi (NATIONALLICENCE)springer-10.1007/BF00172557 A root tissue culture system to study winter wheat-rhizobacteria interactions [Elektronische Daten] [J. de Freitas, James Germida] Summary: A tissue culture procedure was developed for growing winter wheat roots. This system was used to study bacterial-root interactions and root colonization by Pseudomonas cepacia strains R55 and R85, Azospirillum brasilense ATCC 29729 and Azotobacter chroococcum ATCC 9043. Axenic root tissue cultures were inoculated with bacteria and incubated at 25° C on a rotary shaker (150 rpm) for up to 3 weeks. At various intervals, root morphology and root hair development, bacterial colonization of root surfaces and nitrogenase activity were determined. As determined by plate counting techniques, bacterial attachment to the roots varied from 7.5 × 104 to 3.2 × 107 colony-forming units cm−1. Scanning electron microscopy of inoculated roots revealed that some rhizobacteria (e.g., P. cepacia R85) significantly enhanced root hair development, and others (e.g., P. cepacia R55) specifically colonized root exudation sites. Nitrogenase activity of roots inoculated with either A. brasilense ATCC 29729 or A. chroococcum ATCC 9043 was stimulated if the inorganic nitrogen sources in the medium were replaced with 300 μg glutamine ml−1. We propose that the use of root tissue culture systems will facilitate studies on plant root-microbe interactions. Springer-Verlag, 1990 de Freitas J. Department of Soil Science, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut Germida James Department of Soil Science, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 589-595 0175-7598 33:5<589 1990 33 253 https://doi.org/10.1007/BF00172557 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172557 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Freitas J. Department of Soil Science, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Germida James Department of Soil Science, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 589-595 0175-7598 33:5<589 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer