caa a22 4500 465824609 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172542 doi (NATIONALLICENCE)springer-10.1007/BF00172542 Deacetylation of xylans by acetyl esterases of Trichoderma reesei [Elektronische Daten] [K. Poutanen, M. Sundberg, H. Korte, J. Puls] Summary: Two previously purified esterases of Trichoderma reesei were used to study the deacetylation of polymeric, oligomeric and dimeric acetylated xylan fragments. For the first time nearly complete enzymatic deacetylation of polymeric xylan with purified acetyl xylan esterase was demonstrated, resulting in precipitation of the remaining polymer structure. The esterases had very different substrate specifities, one having a preference for high molecular weight substrates and the other showing high activity only towards acetyl xylobiose. The latter enzyme was also regioselective, cleaving off the acetyl substituent only from the C-3 position of the xylopyranose ring. The highest xylose yield from acetylated xylan was obtained by the synergistic action of xylanase, \-xylosidase and acetyl xylan esterase. Springer-Verlag, 1990 Poutanen K. Food Research Laboratory, VTT, Biologinkuja 1, 02150, Espoo, Finland aut Sundberg M. Biotechnical Laboratory, VTT, Tietotie 2, SF-02150, Espoo, Finland aut Korte H. Institut of Wood Chemistry, BFH, Leuschnerstrasse 91, D-2050, Hamburg 80, Federal Republic of Germany aut Puls J. Institut of Wood Chemistry, BFH, Leuschnerstrasse 91, D-2050, Hamburg 80, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 506-510 0175-7598 33:5<506 1990 33 253 https://doi.org/10.1007/BF00172542 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172542 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poutanen K. Food Research Laboratory, VTT, Biologinkuja 1, 02150, Espoo, Finland aut NATIONALLICENCE 700 1- Sundberg M. Biotechnical Laboratory, VTT, Tietotie 2, SF-02150, Espoo, Finland aut NATIONALLICENCE 700 1- Korte H. Institut of Wood Chemistry, BFH, Leuschnerstrasse 91, D-2050, Hamburg 80, Federal Republic of Germany aut NATIONALLICENCE 700 1- Puls J. Institut of Wood Chemistry, BFH, Leuschnerstrasse 91, D-2050, Hamburg 80, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 506-510 0175-7598 33:5<506 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer