caa a22 4500 465824625 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172548 doi (NATIONALLICENCE)springer-10.1007/BF00172548 Molecular cloning, nucleotide sequence and expression in Escherichia coli of the β-cyclodextrin glycosyltransferase gene from Bacillus circulans strain no. 8 [Elektronische Daten] [Lars Nitschke, Karin Heeger, Hans Bender, Georg Schulz] Summary: The β-cyclodextrin glycosyltransferase (β-CGTase) gene was isolated from a λ-library prepared from Bacillus circulans strain no. 8. It was subcloned into plasmid pTZ and expressed by its endogenous regulatory sequences in Escherichia coli JM 103. The structural gene was sequenced and showed an open reading frame for a polypeptide of 718 amino acid residues. The recombinant β-CGTase had the same enzymatic properties as the extracellular CGTase (684 amino acid residues, corresponding to a mol. wt. of 74416) produced by B. circulans strain no. 8. The amino acid sequence showed the highest homology (74.6% identical amino acids) with the CGTase of B. circulans strain F-2, which had been erroneously described as an amylase. The homology with the enzyme from the alkalophilic Bacillus sp. strain no. 1011 was 71.4%. The amino acid sequence derived will be used for elucidating the three-dimensional structure of the enzyme. Springer-Verlag, 1990 Nitschke Lars Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut Heeger Karin Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut Bender Hans Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut Schulz Georg Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 542-546 0175-7598 33:5<542 1990 33 253 https://doi.org/10.1007/BF00172548 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172548 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nitschke Lars Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut NATIONALLICENCE 700 1- Heeger Karin Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bender Hans Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schulz Georg Institut für Organische Chemie und Biochemie, Albertstrasse 21, D-7800, Freiburg im Breisgau, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 542-546 0175-7598 33:5<542 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer