caa a22 4500 46582465X CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172552 doi (NATIONALLICENCE)springer-10.1007/BF00172552 A bifunctional vector system for controlled expression and subsequent release of the cloned gene product by øX174 lysis protein-E [Elektronische Daten] [Udo Bläsi, Stephanie Kalousek, Werner Lubitz] Summary: A new bifunctional Escherichia coli cloning vector, pSB50, is presented. The plasmid allows controlled expression of a gene of interest under control of the lac promoter and the subsequent release of the cloned product by the use of bacteriophage øX174 lysis protein-E, the gene of which is under control of the phage Lambda pL promoter. To ensure optimal repression of the Lambda pL promoter and the lac promoter in plasmid pSB50, E. coli strain UB89-1 was constructed which carries a chromosomal copy of the lambda cl857 repressor allele and the lacl q1 allele, respectively. Here, we employ the E-based lysis system to release human prourokinase to the culture medium. Springer-Verlag, 1990 Bläsi Udo Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Kalousek Stephanie Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Lubitz Werner Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 564-568 0175-7598 33:5<564 1990 33 253 https://doi.org/10.1007/BF00172552 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172552 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bläsi Udo Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Kalousek Stephanie Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Lubitz Werner Institute of Microbiology and Genetics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 564-568 0175-7598 33:5<564 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer