caa a22 4500 465824676 CHVBK 20180323112145.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00172547 doi (NATIONALLICENCE)springer-10.1007/BF00172547 Cloning and expression of cellulase and xylanase genes in Lactobacillus plantarum [Elektronische Daten] [Trees Scheirlinck, Jan De Meutter, Greta Arnaut, Henk Joos, Marc Claeyssens, Frank Michiels] Summary: Eleven cellulase genes from Gram-positive bacteria were cloned in a Lactobacillus plantarum silage inoculum. Eight of these genes were expressed as active enzymes from their original promotors and translation signals. Where tested, the enzymes produced by transformed L.plantarum had the same temperature and pH optimum as enzymes produced in the original host, or in transformed Escherichia coli. Using chloramphenicol acetyltransferase as a cell-internal marker enzyme, it could be demonstrated that at least endoglucanase D from Clostridium thermocellum was actively secreted by transformed L. plantarum. In growing L. plantarum cultures, most of the enzymes were irreversibly inactivated when the pH decreased below 4.5. If the transformed strains were to be applied as an inoculum in silage, this pH inactivation might be useful in preventing overdigestion of the crop fibre. Springer-Verlag, 1990 Scheirlinck Trees Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut De Meutter Jan Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut Arnaut Greta Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut Joos Henk Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut Claeyssens Marc Laboratory for Biochemistry, State University Ghent, Ledeganckstraat 35, B-9000, Ghent, Belgium aut Michiels Frank Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 534-541 0175-7598 33:5<534 1990 33 253 https://doi.org/10.1007/BF00172547 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00172547 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scheirlinck Trees Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- De Meutter Jan Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Arnaut Greta Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Joos Henk Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Claeyssens Marc Laboratory for Biochemistry, State University Ghent, Ledeganckstraat 35, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Michiels Frank Plant Genetic Systems NV, Plateaustraat 22, B-9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/5(1990-08-01), 534-541 0175-7598 33:5<534 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer