caa a22 4500 465824730 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176511 doi (NATIONALLICENCE)springer-10.1007/BF00176511 Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains [Elektronische Daten] [Thomas Homann, Carmen Tag, Hanno Biebl, Wolf-Dieter Deckwer, Bernhard Schink] Summary: Glycerol-fermenting anaerobes were enriched with glycerol at low and high concentrations in order to obtain strains that produce 1,3-propanediol. Six isolates were selected for more detailed characterization; four of them were identified as Citrobacter freundii, one as Klebsiella oxytoca and one as K. pneumoniae. The Citrobacter strains formed 1.3-propanediol and acetate and almost no by-products, while the Klebsiella strains produced varying amounts of ethanol in addition and accordingly less 1,3-propanediol. Enterobacterial strains of the genera Enterobacter, Klebsiella, and Citrobacter from culture collections showed similar product patterns except for one group which formed limited amounts of ethanol, but no propanediol. Seven strains were grown in pH-controlled batch cultures to determine the parameters necessary to evaluate their capacity for 1,3-propanediol production. K. pneumoniae DSM 2026 exhibited the highest final concentration (61 g/l) and the best productivity (1.7 g/l h) whereas C. freundii Zu and K2 achieved only 35 g/l and 1.4 g/l h, respectively. The Citrobacter strains on the other hand gave somewhat better yields which were very close to the theoretical optimum of 65 mol %. Springer-Verlag, 1990 Homann Thomas GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Tag Carmen GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Biebl Hanno GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Deckwer Wolf-Dieter GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut Schink Bernhard Lehrstuhl für Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 121-126 0175-7598 33:2<121 1990 33 253 https://doi.org/10.1007/BF00176511 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176511 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Homann Thomas GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Tag Carmen GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Biebl Hanno GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Deckwer Wolf-Dieter GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schink Bernhard Lehrstuhl für Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 121-126 0175-7598 33:2<121 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer