caa a22 4500 465824749 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176522 doi (NATIONALLICENCE)springer-10.1007/BF00176522 Biosynthesis of aromatic amino acids in Nocardia sp. 239: effects of amino acid analogues on growth and regulatory enzymes [Elektronische Daten] [L. de Boer, G. Grobben, J. Vrijbloed, L. Dijkhuizen] Summary: Further steps required for overproduction of aromatic amino acids by a mutant strain of Nocardia sp. 239 (Noc 87-13), unable to grow on l-phenylalanine as a sole carbon and energy source, were investigated. A number of analogues of the aromatic amino acids displayed severe inhibitory effects on the activities of regulatory enzymes in the biosynthetic pathway and growth of the organism in glucose mineral medium. l-Tryptophane analogues strongly inhibited 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase activity. l-Tyrosine analogues especially inhibited DAHP synthase and chorismate mutase, whereas l-phenylalanine analogues strongly inhibited chorismate mutase and prephenate dehydratase activity. Addition of the aromatic amino acids and their precursors chorismate, 4-hydroxyphenylpyruvate, phenylpyruvate and anthranilate, to the medium counteracted the growth inhibitory effect of specific analogues. The data indicate that ortho- (OFP) and para-fluoro-d,l-phenylalanine (PFP), and l-phenylalanine amide, are the most suitable analogues for the isolation of feedback-inhibition-insensitive prephenate dehydratase mutants. Attempts to isolate l-tyrosine and l-trytophane auxotrophic mutants were only successful in the latter case, resulting in the selection of a stable anthranilate synthase-negative mutant (Noc 87-13-14). Uptake of aromatic amino acids in Nocardia sp. 239 most likely involves a common transport system. This necessitates the use of anthranilate, rather than l-trytophane, as a supplement during the isolation of l-tyrosine auxotrophic and OFP- and/or PFP-resistant mutant derivative strains of Noc 87-13-14. Springer-Verlag, 1990 de Boer L. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut Grobben G. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut Vrijbloed J. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut Dijkhuizen L. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 183-189 0175-7598 33:2<183 1990 33 253 https://doi.org/10.1007/BF00176522 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176522 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Boer L. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut NATIONALLICENCE 700 1- Grobben G. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut NATIONALLICENCE 700 1- Vrijbloed J. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut NATIONALLICENCE 700 1- Dijkhuizen L. Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 183-189 0175-7598 33:2<183 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer