caa a22 4500 465824765 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176521 doi (NATIONALLICENCE)springer-10.1007/BF00176521 Lipid production of revertants of Ufa mutants from the oleaginous yeast Apiotrichum curvatum [Elektronische Daten] [Adrie Ykema, Elizabeth Verbree, Ira Verwoert, Karin van der Linden, H. Nijkamp, Henk Smit] Summary: From six unsaturated fatty acid auxotrophs (Ufa mutants) of the oleaginous yeast Apiotrichum curvatum blocked in the conversion of stearic to oleic acid, were isolated revertants able to grow in the absence of unsaturated fatty acids, in a search for strains that can produce cocoa butter equivalents. A broad range in the percentage of saturated fatty acids (%SFA) was observed in the lipids of individual revertants (varying from 27%-86% SFA), compared with the wild-type (44% SFA). Further analysis of fatty acid composition indicated that: (i) not all six Ufa mutants had the same genetic background and (ii) one specific Ufa mutation could be reverted in more than one way. Revertants that produced lipids with a %SFA>56%, were examined further. These strains were cultivated for 50 generations and half of them produced lipids with high %SFA after that time and were defined as stable. The viability of revertant strains with extremely high %SFA (>80%) may be explained by our finding that polar lipids, which are part of yeast membranes, contained much more polyunsaturated fatty acids and a significantly lower %SFA than neutral (storage) lipids. One revertant (R25.75) was selected that was able to produce lipids in whey permeate at a rate comparable with wild-type A. curvatum and with a fatty acid composition and congelation curve comparable with cocoa butter. Springer-Verlag, 1990 Ykema Adrie Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut Verbree Elizabeth Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut Verwoert Ira Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut van der Linden Karin Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut Nijkamp H. Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut Smit Henk Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 176-182 0175-7598 33:2<176 1990 33 253 https://doi.org/10.1007/BF00176521 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176521 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ykema Adrie Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Verbree Elizabeth Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Verwoert Ira Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van der Linden Karin Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Nijkamp H. Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Smit Henk Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 176-182 0175-7598 33:2<176 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer