caa a22 4500 46582479X CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176530 doi (NATIONALLICENCE)springer-10.1007/BF00176530 Characterization of a major xylanase purified from Lentinula edodes cultures grown on a commercial solid lignocellulosic substrate [Elektronische Daten] [Chittra Mishra, Ian Forrester, Brian Kelley, Richard Burgess, Gary Leatham] Summary: The white-rot basidiomycete Lentinula (syn. Lentinus) edodes (Berk.) Pegler is the dominant edible mushroom cultivated on wood. The major xylanase detected in cultures grown on a commercial oak wood medium was extracted, purified, and characterized. The enzyme was a non-debranching endo-β-d-xylanase (1,4-β-d-xylan xylanohydrolase; E.C.3.2.1.8) highly specific for xylans, with a molecular weight of 41 000 (on sodium dodecyl sulfate gels) and an isoelectric point of 3.6. With aspen glucuronoxylan as substrate, the enzyme showed optimal activity at pH 4.5-5.0 and 60° C, with a K m of 0.66 mg/ml and specific activity of 310 units/mg protein at 40° C. It was capable of hydrolyzing (forming reducing sugars from) 40%-50% of the hydrolyzable linkages in either glucuronoxylan or arabinoxylan. The enzyme produced xylose and major identifiable products in the xylobiose or xylotriose (and presumably larger) size range including xylobiose and xylooligosaccharides, but neither glucuronic acid nor arabinose. Products were also produced from arabinoxylan that appeared to be arabinoxylobiose and arabinoxylotriose. Springer-Verlag, 1990 Mishra Chittra Repligen-Sandoz Research Corporation, Lexington, Mass, USA aut Forrester Ian The Biotechnology Center, University of Wisconsin, Madison, Wisc, USA aut Kelley Brian The Biotechnology Center, University of Wisconsin, Madison, Wisc, USA aut Burgess Richard The Biotechnology Center, University of Wisconsin, Madison, Wisc, USA aut Leatham Gary USDA Forest Service, Forest Products Laboratory, Institute for Microbial and Biochemical Technology, One Pinchot Drive, 53 705-2398, Madison, WI, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 226-232 0175-7598 33:2<226 1990 33 253 https://doi.org/10.1007/BF00176530 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176530 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mishra Chittra Repligen-Sandoz Research Corporation, Lexington, Mass, USA aut NATIONALLICENCE 700 1- Forrester Ian The Biotechnology Center, University of Wisconsin, Madison, Wisc, USA aut NATIONALLICENCE 700 1- Kelley Brian The Biotechnology Center, University of Wisconsin, Madison, Wisc, USA aut NATIONALLICENCE 700 1- Burgess Richard The Biotechnology Center, University of Wisconsin, Madison, Wisc, USA aut NATIONALLICENCE 700 1- Leatham Gary USDA Forest Service, Forest Products Laboratory, Institute for Microbial and Biochemical Technology, One Pinchot Drive, 53 705-2398, Madison, WI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 226-232 0175-7598 33:2<226 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer