caa a22 4500 46582482X CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176532 doi (NATIONALLICENCE)springer-10.1007/BF00176532 Kinetic studies of acetate fermentation by Methanosarcina sp. MSTA-1 [Elektronische Daten] [M. Clarens, R. Molleta] Summary: The effect of three parameters (initial acetate concentration, temperature and pH) on the acetoclastic reaction was studied with the thermophilic methanogenic bacterium Methanosarcina sp. MSTA-1. The optimum temperature for growth ranged around 55° C, and optimum pH was 6.5-7.5, giving a minimum generation time of 12.6-13.9 h (µmax = 0.050-0.055 h−1) and a maximum value of the specific acetate consumption rate (q infs supps ) of 14-20 mmol/g cells per hour. Contrary to the methane yield, the growth yield was found to be dependent on culture conditions, especially on incubation temperature. Methanosarcina sp. MSTA-1 showed a low affinity for acetate substrate. Growth at 55° C and at constant pH 7 resulted in a K m value and a threshold acetate concentration of 10.7 mM and 0.7 mM, respectively. Springer-Verlag, 1990 Clarens M. Laboratoire de Biotechnologie de l'Environnement des Industries Agroalimentaires, Institut National de la Recherche Agronomique, Boulevard du General de Gaulle, BP 429, F-11 104, Narbonne Cedex, France aut Molleta R. Laboratoire de Biotechnologie de l'Environnement des Industries Agroalimentaires, Institut National de la Recherche Agronomique, Boulevard du General de Gaulle, BP 429, F-11 104, Narbonne Cedex, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 239-244 0175-7598 33:2<239 1990 33 253 https://doi.org/10.1007/BF00176532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clarens M. Laboratoire de Biotechnologie de l'Environnement des Industries Agroalimentaires, Institut National de la Recherche Agronomique, Boulevard du General de Gaulle, BP 429, F-11 104, Narbonne Cedex, France aut NATIONALLICENCE 700 1- Molleta R. Laboratoire de Biotechnologie de l'Environnement des Industries Agroalimentaires, Institut National de la Recherche Agronomique, Boulevard du General de Gaulle, BP 429, F-11 104, Narbonne Cedex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 239-244 0175-7598 33:2<239 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer