caa a22 4500 465824838 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176513 doi (NATIONALLICENCE)springer-10.1007/BF00176513 Growth and hyoscyamine production of ‘hairy root' cultures of Datura stramonium in a modified stirred tank reactor [Elektronische Daten] [M. Hilton, M. Rhodes] Summary: The growth and hyoscyamine production of transformed roots of Datura stramonium have been examined in a modified 14-1 stirred tank reactor in both batch and continuous fermentations on media containing half or full strength Gamborg's B5 salts and at three different temperatures. Under a range of conditions, roots grown on half strength B5 salts with 3% w/v sucrose had a higher dry matter content (up to 8.3% w/w) and a higher hyoscyamine content (up to 0.52 mg·g−1 wet weight) than roots grown on full strength B5 salts with the same level of sucrose (up to 4.6% w/w dry matter and up to 0.33 mg hyoscyamine g−1 wet weight). Growth at 30°C was initially faster than at either 25°C or 35°C and by day 12, the drained weight of roots in the fermentor at 30°C was about fourfold greater than at 25°C and twice that at 35°C. The ultimate hyoscyamine levels attained (approximately 0.5 mg·g−1 wet weight) were similar at both 25°C and 30°C but some 40% lower at 35°C. Final packing densities of 70% w/v were achieved for roots after 37 days growth at 25°C and the highest production rate of 8.2 mg hyoscyamine l−1 per day was obtained for roots grown at 30°C. In continuous fermentation at 25°C, the release of hyoscyamine into the culture medium was low (less than 0.5% w/w of the total) but was up to sevenfold higher in fermentors operated at 30°C or 35°C. Springer-Verlag, 1990 Hilton M. Plant Cell Biotechnology Group, AFRC Institute of Food Research, (Norwich Laboratory), Colney Lane, NR4 7UA, Norwich, UK aut Rhodes M. Plant Cell Biotechnology Group, AFRC Institute of Food Research, (Norwich Laboratory), Colney Lane, NR4 7UA, Norwich, UK aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 132-138 0175-7598 33:2<132 1990 33 253 https://doi.org/10.1007/BF00176513 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176513 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hilton M. Plant Cell Biotechnology Group, AFRC Institute of Food Research, (Norwich Laboratory), Colney Lane, NR4 7UA, Norwich, UK aut NATIONALLICENCE 700 1- Rhodes M. Plant Cell Biotechnology Group, AFRC Institute of Food Research, (Norwich Laboratory), Colney Lane, NR4 7UA, Norwich, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 132-138 0175-7598 33:2<132 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer