caa a22 4500 465824854 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176524 doi (NATIONALLICENCE)springer-10.1007/BF00176524 Influence of inorganic phosphate and immobilization on Claviceps purpurea [Elektronische Daten] [Michael Lohmeyer, Wilfried Dierkes, Hans-Jürgen Rehm] Summary: The influence of inorganic phosphate and immobilization on cells of Claviceps purpurea strain 1029/N5 producing ergot peptides in shake culture was examined. Immobilization in Ca-alginate beads resulted in a marked reduction of some metabolic activities, i.e. the periods of alkaloid formation and cell growth were prolonged. High concentrations of inorganic phosphate (1 g/l KH2PO4) could reduce or stop alkaloid formation both by free and immobilized cells at any time during fermentation. The optimum phosphate concentration for alkaloid production by immobilized cells (about 0.5 mM) was a quarter of that required by free cells. This optimum shift was attributed to (i) the diminished phosphate demand of immobilized cells, due to their reduced metabolic activities, and (ii) the phosphate-dependent morphological behaviour of the biocatalyst. The observed decrease in alkaloid concentrations during later periods of the fermentation supported the idea of alkaloid-degradative enzymes, activated by high phosphate concentrations. Immobilization showed an advantageous influence on this undesirable effect. Springer-Verlag, 1990 Lohmeyer Michael Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Federal Republic of Germany aut Dierkes Wilfried Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Federal Republic of Germany aut Rehm Hans-Jürgen Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 196-201 0175-7598 33:2<196 1990 33 253 https://doi.org/10.1007/BF00176524 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176524 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lohmeyer Michael Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Dierkes Wilfried Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Rehm Hans-Jürgen Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 196-201 0175-7598 33:2<196 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer