caa a22 4500 465824862 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176523 doi (NATIONALLICENCE)springer-10.1007/BF00176523 Molecular breeding of a Brevibacterium lactofermentum l -phenylalanine producer using a cloned prephenate dehydratase gene [Elektronische Daten] [Hisao Ito, Katsuaki Sato, Kazuhiko Matsui, Konosuke Sano, Hitoshi Enei, Yoshio Hirose] Summary: The prephenate dehydratase gene was cloned from a mutant of Brevibacterium lactofermentum, AJ11957 that produced enzyme free from feedback inhibition. The recombinant plasmids pPH11 and pPH14 complemented a phenylalanine auxotroph of B. lactofermentum, A-15, provided the transformant with the desensitized enzyme and caused an increased level of the enzyme compared to that of a wild strain. Plasmid pPH14 was introduced into l-phenylalanine producers genetically induced from B. lactofermentum; MF358 and FP-1 excreting l-tyrosine and anthranilate, respectively, as by-products. Both transformants predominantly accumulated l-phenylalanine at the expense of by-product formation. Co-existence of pPH14 and pTAR16, a recombinant plasmid expressing desensitized 3-deoxy-d-arabino-hepturosonate-7-phosphate synthase had a marked effect on further improvement in l-phenylalanine productivity, accompanied by an increase in the corresponding enzyme activity. The parent, MF358, accumulating 5.5 g/l l-phenylalanine, 6.8 g/l l-tyrosine and 0.3 g/l anthranilate turned into a potent l-phenylalanine producer producing 18.2 g/l l-phenylalanine and 1.0 g/l l-tyrosine by-product. Springer-Verlag, 1990 Ito Hisao Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut Sato Katsuaki Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut Matsui Kazuhiko Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut Sano Konosuke Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut Enei Hitoshi Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut Hirose Yoshio Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 190-195 0175-7598 33:2<190 1990 33 253 https://doi.org/10.1007/BF00176523 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176523 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ito Hisao Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut NATIONALLICENCE 700 1- Sato Katsuaki Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut NATIONALLICENCE 700 1- Matsui Kazuhiko Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut NATIONALLICENCE 700 1- Sano Konosuke Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut NATIONALLICENCE 700 1- Enei Hitoshi Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut NATIONALLICENCE 700 1- Hirose Yoshio Process Development Laboratory, Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, 210, Kawasaki-ku, Kawasaki-shi, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 190-195 0175-7598 33:2<190 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer