caa a22 4500 465824897 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176519 doi (NATIONALLICENCE)springer-10.1007/BF00176519 Diffusion in immobilized-cell agar layers: influence of bacterial growth on the diffusivity of potassium chloride [Elektronische Daten] [Laurent Mignot, Guy-Alain Junter] Summary: The diffusitivity of potassium chloride in composite agar slab/microporous membrane structures loaded with various amounts of Escherichia coli whole cells was determined using both time-lag and steady-state methods. The diffusion coefficient of KCl decreased linearly with the logarithm of the immobilized-cells content. The effect exerted by bacterial growth inside the immobilization matrices on KCl diffusivity was then investigated. The diffusion coefficient of KCl obtained by time-lag analysis decreased during incubation of the immobilized-cell structures, whereas less consitent results arose from the steady-state method. An apparent doubling time for immobilized E. coli, increasing with the initial cell content of the gel, was obtained from the calibration relationship between KCl diffusivity and the number of organisms in agar. Springer-Verlag, 1990 Mignot Laurent Laboratory of Macromolecular Chemistry, URA CNRA No. 500, University of Rouen, PO Box 118, F-76134, Mont-Saint-Aignan Cédex, France aut Junter Guy-Alain Laboratory of Macromolecular Chemistry, URA CNRA No. 500, University of Rouen, PO Box 118, F-76134, Mont-Saint-Aignan Cédex, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 167-171 0175-7598 33:2<167 1990 33 253 https://doi.org/10.1007/BF00176519 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176519 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mignot Laurent Laboratory of Macromolecular Chemistry, URA CNRA No. 500, University of Rouen, PO Box 118, F-76134, Mont-Saint-Aignan Cédex, France aut NATIONALLICENCE 700 1- Junter Guy-Alain Laboratory of Macromolecular Chemistry, URA CNRA No. 500, University of Rouen, PO Box 118, F-76134, Mont-Saint-Aignan Cédex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 167-171 0175-7598 33:2<167 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer