caa a22 4500 465824919 CHVBK 20180323112146.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00176516 doi (NATIONALLICENCE)springer-10.1007/BF00176516 The use of free and immobilised Arthrobacter simplex in organic solvent/aqueous two-liquid-phase reactors [Elektronische Daten] [M. Hocknull, M. Lilly] Summary: The use of free and immobilised Arthrobacter simplex (NCIB 8929) for steroid Δ1-dehydrogenation in two-liquid-phase, stirred-tank reactors has been compared. Product formation is related to the logarithm of the water-octanol partition coefficient (log P) of the organic solvent employed, but the relationship is different for the two forms of the biocatalyst. No reaction was seen with either biocatalyst in media containing solvents of log P≤2.5. For free bacteria, product formation rose linearly with log P thereafter to a maximum at a value of 9.8. With immobilised bacteria, product formation reached a maximum with a solvent of log P=4.0 and remained constant with solvents of higher log P value. Consequently extended reactor operation was possible with immobilised bacteria, and the production of high quality (> 95% purity) steroid product was demonstrated. Springer-Verlag, 1990 Hocknull M. SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, United Kingdom aut Lilly M. SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, United Kingdom aut Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 148-153 0175-7598 33:2<148 1990 33 253 https://doi.org/10.1007/BF00176516 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176516 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hocknull M. SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, United Kingdom aut NATIONALLICENCE 700 1- Lilly M. SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, United Kingdom aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/2(1990-05-01), 148-153 0175-7598 33:2<148 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer