caa a22 4500 465826172 CHVBK 20180323112149.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00691716 doi (NATIONALLICENCE)springer-10.1007/BF00691716 Anaerobic energy metabolism in the oligochaete Lumbriculus variegatus Müller [Elektronische Daten] [V. Putzer, A. de Zwaan, W. Wieser] Summary: Anoxia tolerance, glycogen degradation, free amino acid pool, adenylate energy charge and the accumulation and excretion of end products were monitored inLumbriculus variegatus Müller throughout 48 h of anoxia. A transition period lasting about 4 h could be distinguished from subsequent events during which malate, present in high amounts in the resting animals, is utilized, probably by conversion to succinate. Up to the 12th hour of anoxia there is an increase in concentration of free amino acids, except aspartate. Glutamate increases rapidly during the first half hour but decreases thereafter. Beginning with the second hour of anoxia the alanine concentration increases at the same rate glutamate concentration decreases, but the source of nitrogen during the first hour is unknown. It is argued that the nitrogen required for the synthesis of some of the amino acids is ultimately derived from proteolysis. After about 3 h of anoxia propionate and acetate are synthesized. At first these acids accumulate in the tissues, but after 4-6 h they are excreted into the surrounding medium. Acetate is excreted over the whole experimental period at a constant rate, whereas the excretion rate of propionate decreases slowly with time. The propionate/acetate ratio is in excess of 2. Classic malate dismutation is by far the most important mechanism in the maintenance of redox balance. Depletion of glycogen stores appears to play an important role in determining anoxic survival time. Due to extremely low activity of PEPCK the ratio of the specific activities of PK and PEPCK is very high. Further, the kinetic properties of pyruvate kinase do not support the assumption of a shift of the glycolytic carbon flow at the PEP level. Springer-Verlag, 1990 Lumbriculus variegatus nationallicence anoxia tolerance nationallicence glycogen nationallicence free amino acids nationallicence metabolites of anaerobic metabolism nationallicence malate dismutation nationallicence PK : Pyruvate kinase nationallicence PEPCK : phosphoenolpyruvate carboxykinase nationallicence PEP : phospho(enol)pyruvate nationallicence FBP : fructose-1,6-bisphosphate nationallicence AEC : adenylate energy charge nationallicence EMP-scheme : Embden-Meyerhof-Parnas scheme of glycolysis nationallicence f w : fresh body weight nationallicence dw : dry body weight nationallicence Putzer V. Institut für Zoologie, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria aut de Zwaan A. Institut für Zoologie, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria aut Wieser W. Institut für Zoologie, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria aut Journal of Comparative Physiology B Springer-Verlag 159/6(1990-11-01), 707-715 0174-1578 159:6<707 1990 159 360 https://doi.org/10.1007/BF00691716 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00691716 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Putzer V. Institut für Zoologie, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- de Zwaan A. Institut für Zoologie, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Wieser W. Institut für Zoologie, Universität Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 159/6(1990-11-01), 707-715 0174-1578 159:6<707 1990 159 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer