caa a22 4500 465826180 CHVBK 20180323112149.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00691711 doi (NATIONALLICENCE)springer-10.1007/BF00691711 Nestler James Department of Environmental, Population, and Organismic Biology, University of Colorado, 80309, Boulder, Colorado, USA aut Intracellular pH during daily torpor in Peromyscus maniculatus [Elektronische Daten] [James Nestler] Summary: Intracellular and extracellular acid-base parameters during normothermy and daily torpor were examined in deer mice (Peromyscus maniculatus). [14C]Dimethyloxazolidinedione and [3]inulin were used to assess intracellular pH in liver, heart, skeletal muscle, and brain. Buffering capacities were determined using tissue homogenates. A significant increase in plasma $$P_{{\text{CO}}_2 } $$ and $$C_{{\text{CO}}_2 } $$ during daily torpor indicates a respiratory acidosis. All tissues experienced a reduction in the calculated dissociation ratio of histidine imidazole groups (αimid) during daily torpor (16.5% for brain, approximately 10% for other tissues). Based on comparisons with physicochemical tissue buffering capacities, metabolic compensation of the respiratory acidosis occurred in liver, heart, and plasma, while brain was more acidotic than predicted. The more extensive change in brain αimid might influence a regulated decrease in body temperature. Comparison of acid-base parameters during daily torpor and hibernation suggests that the magnitude of acid-base modifications in mammals may be associated with the level of dormancy. Springer-Verlag, 1990 Daily torpor nationallicence Imidazole nationallicence Acid-base balance nationallicence Buffering capacity nationallicence αimid : dissociation ratio of histidine imidazole groups nationallicence β : physicochemical non-bicarbonate buffer value nationallicence β' : apparent (in vivo) buffer value nationallicence bicarbonate : bicarbonate values corrected to a temperature of 25 °C nationallicence pH : pH values corrected to a temperature of 25 °C nationallicence pH i : intracellular pH nationallicence pK imid : pK of histidine imidazole groups nationallicence T b : body temperature nationallicence Journal of Comparative Physiology B Springer-Verlag 159/6(1990-11-01), 661-666 0174-1578 159:6<661 1990 159 360 https://doi.org/10.1007/BF00691711 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00691711 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Nestler James Department of Environmental, Population, and Organismic Biology, University of Colorado, 80309, Boulder, Colorado, USA aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 159/6(1990-11-01), 661-666 0174-1578 159:6<661 1990 159 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer