caa a22 4500 465826210 CHVBK 20180323112150.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00691713 doi (NATIONALLICENCE)springer-10.1007/BF00691713 Neural regulation of the pulmonary vasculature in a semi-arboreal snake, Elaphe obsoleta [Elektronische Daten] [John Donald, James O'Shea, Harvey Lillywhite] Summary: The innervation of the pulmonary vasculature of the semi-arboreal rat snake,Elaphe obsoleta, was examined with glyoxylic acid-induced catecholamine histochemistry, peptide immunohistochemistry, and in vitro perfusion of the pulmonary vasculature. An adrenergic innervation was present on the pulmonary artery, the smaller pulmonary arteries, the veins draining the lung, and the main pulmonary vein. Vasoactive intestinal polypeptide-like immunoreactive axons were observed on the pulmonary artery and vein, small arteries, and occasionally small veins within the lung parenchyma. A dense plexus of substance P-like immunoreactive (SP-LI) axons was observed on the distal extrinsic pulmonary artery. SP-LI axons were found on the more distal arteries within the lung parenchyma, but not on the veins. The distribution of calcitonin gene-related peptide- and SP-LI axons was similar suggesting that the axons are sensory nerves. In the perfused pulmonary vasculature, vagal stimulation caused a predominant vasoconstriction which was abolished by atropine indicating it was cholinergic in nature. A post-stimulus vasodilatation was abolished by bretylium and propranolol indicating it was adrenergic in nature. The responses to nerve stimulation were located in both the extrinsic and intrinsic pulmonary vasculature. No evidence for non-adrenergic, noncholinergic transmission to the vascular smooth muscle was found. The extensive, functional innervation of the main pulmonary artery, as well as the more distal vasculature within the lung, may reflect adaptation to cardiovascular problems imposed by an elongated body and arboreal habits. Springer-Verlag, 1990 Pulmonary vasculature nationallicence Vagus nerve nationallicence Neuropeptides nationallicence Catecholamines nationallicence Snake nationallicence VIP : vasoactive intestinal polypeptide nationallicence VIP-LI : vasoactive intestinal polypeptide-like immunoreactive nationallicence SP : substance P nationallicence SP-LI : substance P-like immunoreactive nationallicence SOM : somatostatin nationallicence SOM-LI : somatostatin-like immunoreactive nationallicence CGRP : calcitonin gene-related peptide nationallicence CGRP-LI : calcitonin gene-related peptide-like immunoreactive nationallicence NANC : non-adrenergic noncholinergic nationallicence PI : perfusion inflow nationallicence Donald John Department of Zoology, University of Florida, 32611, Gainesville, Florida, USA aut O'Shea James Department of Zoology, University of Western Australia, 6009, Nedlands, Western Australia, Australia aut Lillywhite Harvey Department of Zoology, University of Florida, 32611, Gainesville, Florida, USA aut Journal of Comparative Physiology B Springer-Verlag 159/6(1990-11-01), 677-685 0174-1578 159:6<677 1990 159 360 https://doi.org/10.1007/BF00691713 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00691713 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donald John Department of Zoology, University of Florida, 32611, Gainesville, Florida, USA aut NATIONALLICENCE 700 1- O'Shea James Department of Zoology, University of Western Australia, 6009, Nedlands, Western Australia, Australia aut NATIONALLICENCE 700 1- Lillywhite Harvey Department of Zoology, University of Florida, 32611, Gainesville, Florida, USA aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 159/6(1990-11-01), 677-685 0174-1578 159:6<677 1990 159 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer