caa a22 4500 465826377 CHVBK 20180323112150.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00258768 doi (NATIONALLICENCE)springer-10.1007/BF00258768 Pheromone-induced stimulation of inositol-trisphosphate formation in insect antennae is mediated by G-proteins [Elektronische Daten] [I. Boekhoff, K. Raming, H. Breer] Summary: Phospholipase C in the antennae of Periplaneta americana was stimulated by pheromones in a GTP-dependent manner. The enzyme activity was potentiated by hydrolysis-resistant analogs of GTP and decreased by GDP analogs. Guanine nucleotide binding regulatory proteins (G-proteins) in antennal preparations were identified by bacterial toxin-catalyzed ADP-ribosylation and immunoreactivity with antibodies of designed specificity. The stimulatory effect of pheromones on inositol phosphate formation was completely blocked by pertussis toxin suggesting that the pheromone action was mediated via specific G-proteins. Springer-Verlag, 1990 Phospholipase nationallicence G-protein nationallicence Periplaneta nationallicence Periplanone nationallicence Olfaction nationallicence G-protein : guanine nucleotide-binding regulatory protein nationallicence IP 3 : inositol 1,4,5-trisphosphate nationallicence GTPS : guanine-5′-[thio]-triphosphate nationallicence GDP β S : guanine-5′-[β-thio]-diphosphate nationallicence NAD : nicotinic acid adenine dinucleotide nationallicence SDS-PAGE : sodium dodecylsulfate polyacrylamide gel electrophoresis nationallicence DTT : dithiothreitol nationallicence MOPS : 3-(N-morpholino)propanesulfonic acid nationallicence PIPES : piperazine-N,N′-bis[2-ethanesulfonic acid] nationallicence DMSO : dimethyl-sulfoxide nationallicence Boekhoff I. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany aut Raming K. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany aut Breer H. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany aut Journal of Comparative Physiology B Springer-Verlag 160/1(1990-04-01), 99-103 0174-1578 160:1<99 1990 160 360 https://doi.org/10.1007/BF00258768 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00258768 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boekhoff I. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany aut NATIONALLICENCE 700 1- Raming K. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany aut NATIONALLICENCE 700 1- Breer H. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 160/1(1990-04-01), 99-103 0174-1578 160:1<99 1990 160 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer