caa a22 4500 465826458 CHVBK 20180323112150.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00258761 doi (NATIONALLICENCE)springer-10.1007/BF00258761 Comparative glucose tolerance studies in the freshwater snail Biomphalaria glabrata [Elektronische Daten] influence of starvation and infection with the trematode Schistosoma mansoni [Manfred Liebsch, Wilhelm Becker] Summary: The dynamic reactions of B. glabrata on intravasal loads of 20-1000 μg glucose·g live fresh wt-1 were studied in standard fed snails (SFS). Starved snails (SS) and snails infected with S. mansoni (IS) were given 100-500 μg glucose·g fresh live wt-1. Mean hemolymph glucose level was 12.1 mg·100 ml-1 in SFS. It was not significantly lower in SS (10.7 mg·100 ml-1), but significantly reduced in IS (8.5 mg·100 ml-1). Since hemolymph volumes were significantly increased in SS, the amount of circulating glucose (pool) did not change (75 μg·g body weight-1) compared to SFS (62 μg·g-1). It was, however, reduced to 41 μg·g-1 in IS. In SFS the circulating glucose pool had to be doubled to induce significantly elimination of the injected glucose. Tripled pools were eliminated with half-times of 45 min, whereas lower and higher glucose loads were eliminated significantly slower (half-times: 80-105 min). Glucose tolerance of SS was reduced: half-times were doubled, and metabolization of injected glucose was reduced. Since tissue fresh weights were lowered by 40%, absolute incorporation of 14C from labeled glucose was lowered, but specific incorporation (per mg) was higher than in SFS and IS. Glucose tolerance of IS was increased: metabolic clearance rates rose by 70% and half-times were shortened by 30%, though absolute and specific rates of 14C incorporation were lowered. However, IS lost 25% of the label to the water, whereas SFS lost 12% and SS only lost 8%. Using the antimetabolite 2-deoxyglucose, 80% of the losses proved to be glucose in IS, and 50% in SFS. The present results suggest the existence of a glucostatic regulation in B. glabrata with lower sensitivity and capacity than in mammals. As to glucose tolerance, the often reported parallelism in metabolic shifts induced by starvation and parasitic infection was not confirmed. Springer-Verlag, 1990 Glucose tolerance nationallicence Glucose pool nationallicence Starvation nationallicence Infection nationallicence Anaesthesia nationallicence SFS : standard fed snails nationallicence SS : starved snails nationallicence IS : infected snails nationallicence MGG : midgut-gland-gonad nationallicence RB : rest of the bodymass nationallicence MCR : metabolic clearance rate nationallicence Liebsch Manfred Zoologisches Institut und Museum der Universität Hamburg, Martin-Luther-King-Platz 3, D-2000, Hamburg 13, Germany aut Becker Wilhelm Zoologisches Institut und Museum der Universität Hamburg, Martin-Luther-King-Platz 3, D-2000, Hamburg 13, Germany aut Journal of Comparative Physiology B Springer-Verlag 160/1(1990-04-01), 41-50 0174-1578 160:1<41 1990 160 360 https://doi.org/10.1007/BF00258761 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00258761 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liebsch Manfred Zoologisches Institut und Museum der Universität Hamburg, Martin-Luther-King-Platz 3, D-2000, Hamburg 13, Germany aut NATIONALLICENCE 700 1- Becker Wilhelm Zoologisches Institut und Museum der Universität Hamburg, Martin-Luther-King-Platz 3, D-2000, Hamburg 13, Germany aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 160/1(1990-04-01), 41-50 0174-1578 160:1<41 1990 160 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer