caa a22 4500 465826601 CHVBK 20180323112151.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01075664 doi (NATIONALLICENCE)springer-10.1007/BF01075664 Skeletal muscle pH dynamics during arousal from hibernation measured by31P NMR spectroscopy [Elektronische Daten] [M. McArthur, C. Hanstock, A. Malan, L. Wang, P. Allen] Summary: The time-course of changes in skeletal muscle pH during arousal from hibernation in the Columbian ground squirrel was studied using31P NMR spectroscopy. In hibernation (T re 7-9°C), shoulder/neck muscle pH was 7.45±0.03 and αIm was 0.60. In euthermia (T re 37°C), muscle pH was 7.24±0.05 and αIm was 0.75. Thus the overall pH-temperature coefficient was-0.009 pH units/°C, indicating acidification of the muscle in hibernation. During the transition from hibernation to euthermia, however, the muscle shows a nonlinear pattern of pH change. In early arousal (T sh<20-25°C,T re<15°C) muscle pH does not change and muscle αIm increases to 0.72. In later arousal (T sh>20-25°C,T re>15°C) muscle pH decreases gradually toward the euthermic value and muscle αIm increases only slightly from 0.72 to 0.75. These results support the hypothesis that intracellular acidification of the muscle, present during hibernation, is reversed in early arousal. This may facilitate an increase in muscle metabolism and the contribution of maximal shivering thermogenesis to rewarming of the animal. Springer-Verlag, 1990 Ground squirrel nationallicence 31Phosphorus NMR nationallicence Skeletal muscle pH nationallicence Alpha-imidazole nationallicence Arousal from hibernation nationallicence αIm : dissociation ratio of protein imidazole buffergroups nationallicence NST : non-shivering thermogenesis nationallicence BAT : brown adipose tissue nationallicence dp H/dT : temperature coefficient of pH nationallicence pH i : intracellular pH nationallicence 31 P NMR : 31Phosphorus nuclear magnetic resonance nationallicence δ P i : chemical shift of inorganic phosphate relative to PCr nationallicence PCr : phosphocreatine nationallicence T b : body temperature nationallicence T re : rectal temperature nationallicence T sh : subcutaneous shoulder temperature nationallicence T a : ambient temperature nationallicence McArthur M. Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut Hanstock C. Department of Applied Sciences in Medicine, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut Malan A. Laboratoire d'Etude des Régulations Physiologiques (associated with Université Louis Pasteur), CNRS, 67087, Strasbourg, France aut Wang L. Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut Allen P. Department of Applied Sciences in Medicine, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 339-347 0174-1578 160:4<339 1990 160 360 https://doi.org/10.1007/BF01075664 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01075664 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McArthur M. Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Hanstock C. Department of Applied Sciences in Medicine, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Malan A. Laboratoire d'Etude des Régulations Physiologiques (associated with Université Louis Pasteur), CNRS, 67087, Strasbourg, France aut NATIONALLICENCE 700 1- Wang L. Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Allen P. Department of Applied Sciences in Medicine, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 339-347 0174-1578 160:4<339 1990 160 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer