caa a22 4500 465826636 CHVBK 20180323112151.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01075665 doi (NATIONALLICENCE)springer-10.1007/BF01075665 Heat sensitivity and protein synthesis during heat-shock in the tobacco hornworm, Manduca sexta [Elektronische Daten] [Catherine Fittinghoff, Lynn Riddiford] Summary: Fifth instar larvae of the tobacco hornworm,Manduca sexta, tolerate 1-h exposures to temperatures as high as 42°C. Above 42°C, survival declines rapidly to 18% at 44°C and 0% at 48°C. As in other insects, the heat-shock response ofManduca sexta involves the induction of synthesis of heat-shock proteins very similar in size to theDrosophila heat-shock proteins (84, 73, 71, 27, 25, 23, and 22 kd). In the epidermis, heat-shock protein synthesis peaks at 42°C, correlating with the heat sensitivity of both the tissue itself and the intact larva. Some heat-shock proteins have different isoelectric forms depending on tissue. Also, the heat-shock proteins are synthesized over a wider range of temperatures in the imaginal discs and the fat body as compared to the epidermis. In contrast to dipteran insects,Manduca sexta does not exhibit a strong repression of non-heat-shock protein synthesis under tolerable conditions. Springer-Verlag, 1990 Heat sensitivity nationallicence Heat shock nationallicence Protein synthesis Manduca sexta nationallicence Insects nationallicence TCA : trichloroacetic acid nationallicence PAGE : polyacrylamide gel electrophoresis nationallicence AZT : arbitrary Zeitgeber time nationallicence kd : kilodaltons nationallicence Fittinghoff Catherine Department of Zoology, University of Washington, 98195, Seattle, WA, USA aut Riddiford Lynn Department of Zoology, University of Washington, 98195, Seattle, WA, USA aut Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 349-356 0174-1578 160:4<349 1990 160 360 https://doi.org/10.1007/BF01075665 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01075665 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fittinghoff Catherine Department of Zoology, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Riddiford Lynn Department of Zoology, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 349-356 0174-1578 160:4<349 1990 160 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer