caa a22 4500 465826709 CHVBK 20180323112151.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01075668 doi (NATIONALLICENCE)springer-10.1007/BF01075668 Isolation and characterization of red-pigment-concentrating hormone (RPCH) from six crustacean species [Elektronische Daten] [Gabriele Gaus, Lewis Kleinholz, Günter Kegel, Rainer Keller] Summary: Red-pigment-concentrating hormone (RPCH) has been isolated from nerve tissue of six decapod crustaccan species. The primary structure of three of the six hormones, i.e., those ofCancer magister, Carcinus maenas andOrconectes limosus, was determined by manual microsequencing as: pELNFSPGW-NH2. This sequence is identical to that of RPCH fromPandalus borealis, the only previously known sequence of a crustacean RPCH. The other three hormones fromLiocarcinus puber, Nephrops norvegicus, andPacifastacus leniusculus could not be characterized completely. However, amino acid compositions, the presence of N-terminal pGlu, and the blocked N-terminal ends are in accordance with the primary structure established for the other three RPCHs. We suggest that all six peptides have the same amino acid sequence. These results indicate that RPCH, which is likely to be related to the peptides of the AKH family in insects, is highly conserved among crustacean species. This is in remarkable contrast to the high degree of molecular evolution exemplified by the many different AKH-like peptides among insect species. Springer-Verlag, 1990 Red pigment-concentrating hormone nationallicence Crustacean neuropeptide nationallicence Amino acid sequence nationallicence HPLC Chromatophores nationallicence AKH : adipokinetic hormone nationallicence Aufs : absorption units full scale nationallicence BSA : bovine serum albumin nationallicence ECH : erythrophore concentrating hormone (=RPCH) nationallicence EDTA : ethylenediamine-tetra-acetic acid nationallicence HOAc : acetic acid nationallicence HPLC : high pressure liquid chromatography nationallicence O.D. : optical density nationallicence PDH : pigment dispersing hormone nationallicence PGAP : pyroglutamate aminopeptidase nationallicence RPCH : red-pigment-concentrating hormone (=ECH) nationallicence SOG : suboesophageal ganglion nationallicence TFA : trifluoroacetic acid nationallicence Gaus Gabriele Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Federal Republic of Germany aut Kleinholz Lewis Department of Biology, Reed College, 97202, Portland, Oregon, USA aut Kegel Günter Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Federal Republic of Germany aut Keller Rainer Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Federal Republic of Germany aut Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 373-379 0174-1578 160:4<373 1990 160 360 https://doi.org/10.1007/BF01075668 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01075668 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gaus Gabriele Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kleinholz Lewis Department of Biology, Reed College, 97202, Portland, Oregon, USA aut NATIONALLICENCE 700 1- Kegel Günter Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Federal Republic of Germany aut NATIONALLICENCE 700 1- Keller Rainer Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität Bonn, Federal Republic of Germany aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 373-379 0174-1578 160:4<373 1990 160 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer