caa a22 4500 465826717 CHVBK 20180323112151.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01075671 doi (NATIONALLICENCE)springer-10.1007/BF01075671 Nerve impulse-induced release of endogenous noradrenaline and adrenaline from the perfused cod spleen [Elektronische Daten] [F. Ehrenström, A. Ungell] Summary: Release of endogenous catecholamines (CA) by electrical nerve stimulation (NS) was studied in the isolated perfused spleen of the Atlantic cod,Gadus morhua. An HPLC-system for the analysis of endogenously released CA is described. Cocaine (COC) was used to block neuronal re-uptake of endogenous CA released by NS. Splanchnic NS at frequencies of 1-40 Hz for 20 s resulted in release of noradrenaline (NA) and adrenaline (A) with a maximal total overflow at 20 Hz for both amines. The release of CA was frequency-dependent. COC (0.1 mmol·l-1) increased NS-evoked (40 Hz) overflow of NA and A by 4.8 and 2.2 times, respectively, and reduced the overflow of dihydroxyphenylglycol (DOPEG) to spontaneous efflux levels or less. It can be concluded that the HPLC-technique used was adequate for measurement of NS-evoked release of endogenous CA and DOPEG from the isolated perfused cod spleen, and the model presented can therefore be used when studying adrenergic mechanisms in fish spleen. Springer-Verlag, 1990 Endogenous catecholamines nationallicence High performance liquid chromatography nationallicence Stimulation-evoked release nationallicence Spleen nationallicence Teleost nationallicence A : adrenaline nationallicence CA : catecholamines nationallicence COC : cocaine nationallicence DA : dopamine nationallicence DHBA : 3,4-dihydroybenzylamine hydrobromide nationallicence DOPAC : 3,4-dihydroxyphenylacetic acid nationallicence DOPEG : 3,4-dihydroxyphenylglycol nationallicence l - DOPA : 3,4-dihydroxyphenylalanine nationallicence NA : noradrenaline nationallicence NS : nerve stimulation nationallicence PCA : perchloric acid nationallicence Ehrenström F. Comparative Neuroscience Unit, Department of Zoophysiology, University of Göteborg, P.O.B. 250 59, Göteborg, Sweden aut Ungell A. Hassle AB, Department for Drug Delivery Research, Kärragatan 5, S-431 83, Mölndal, Sweden aut Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 401-406 0174-1578 160:4<401 1990 160 360 https://doi.org/10.1007/BF01075671 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01075671 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ehrenström F. Comparative Neuroscience Unit, Department of Zoophysiology, University of Göteborg, P.O.B. 250 59, Göteborg, Sweden aut NATIONALLICENCE 700 1- Ungell A. Hassle AB, Department for Drug Delivery Research, Kärragatan 5, S-431 83, Mölndal, Sweden aut NATIONALLICENCE 773 0- Journal of Comparative Physiology B Springer-Verlag 160/4(1990-07-01), 401-406 0174-1578 160:4<401 1990 160 360 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer